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Purified Mouse Anti-DLP1
Purified Mouse Anti-DLP1

Western blot analysis of DLP1 on a rat cerebrum lysate. 1 µg/mL (lane 1), 0.5 µg/mL (lane 2) and 0.25 µg/mL (lane 3) of the mouse anti-DLP1 antibody.  

Purified Mouse Anti-DLP1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of DLP1 on a rat cerebrum lysate. 1 µg/mL (lane 1), 0.5 µg/mL (lane 2) and 0.25 µg/mL (lane 3) of the mouse anti-DLP1 antibody.  

Immunofluorescence staining of human endothelial cells.

製品詳細
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BD Transduction Laboratories™
Dynamin Like Protein-1; DVLP
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Rat DLP1 aa. 601-722
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
79-84 kDa
250 µg/ml
AB_398424
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611113 Rev. 3
抗体の詳細
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8/DLP1

Dynamins, phosphoproteins with intrinsic GTPase activities, play an important role during early endocytosis.  The GTPase activity is stimulated in vitro by microtubules, SH3 domain containing-proteins, and Protein Kinase (PKC)- mediated phosphorylation.  Although highly related, dynamins are differentially expressed in an organism.  For instance, dynamin I is found only in the nervous system, dynamin II is ubiquitously expressed, and dynamin III is located in lung, testis and brain.  Due to four alternative splicing products for each of the dynamins, the dynamin family contains at least 12 different members.  The Dynamin Like Protein-1 (DLP1) is a new member of the dynamin family of  proteins.  DLP1 (also known as DVLP) is widely expressed as two alternatively spliced products, each with different tissue expression patterns.  The larger product is about 84 kDa and reportedly found only in the brain, whereas the 79 kDa product is ubiquitously expressed.  Unlike dynamins I-III which co-localize with endocytotic vesicles, DLP1 is found aligned with microtubules and with the endoplasmic reticulum, suggesting a secretory function for this novel protein.

611113 Rev. 3
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611113 Rev.3
引用&参考文献
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開発者向け参考資料 (6)

  1. Bossya B, Bossy-Wetzela E, Petrillia A, et al. S-Nitrosylation of DRP1 Does Not Affect Enzymatic Activity and is Not Specific to Alzheimer’s Disease. 2010; 20:S513-S526. (Biology: Western blot). 参考文献を見る
  2. Duvezin-Caubet S, Reichert AS, Jagasia R, et al. Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem. 2006; 281(49):37972-37979. (Biology: Western blot). 参考文献を見る
  3. Estaquier J, Arnoult D. Inhibiting Drp1-mediated mitochondrial fission selectively prevents the release of cytochrome c during apoptosis. Cell Death Differ. 2007; 14(6):1086-1094. (Biology: Western blot). 参考文献を見る
  4. Jofuku A, Ishihara N, Mihara K. Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein. Biochem Biophys Res Commun. 2005; 333(2):650-659. (Biology: Western blot). 参考文献を見る
  5. Karbowski M, Neutzner A, Youle RJ. The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division. J Cell Biol. 2007; 178(1):71-84. (Biology: Immunofluorescence, Western blot). 参考文献を見る
  6. Yoon Y, Pitts KR, Dahan S, McNiven MA. A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. J Cell Biol. 1998; 140(4):779-793. (Biology). 参考文献を見る
すべて表示する (6) 表示項目を減らす
611113 Rev. 3

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