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Purified Mouse Anti-CD51
Purified Mouse Anti-CD51

Western blot analysis of CD51 (Integrin αV) on a rat cerebrum lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti-CD51 antibody.

Purified Mouse Anti-CD51

Immunofluorescence staining of human endothelial cells.

Western blot analysis of CD51 (Integrin αV) on a rat cerebrum lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti-CD51 antibody.

Immunofluorescence staining of human endothelial cells.

製品詳細
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BD Transduction Laboratories™
Integrin αV; Vitronectin Receptor α; VNRα
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1, κ
Human CD51/VNRα aa. 609-722
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
125 kDa
250 µg/ml
AB_2129503
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611012 Rev. 1
抗体の詳細
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21/CD51

Integrins are a family of proteins that mediate intercellular adhesion or adherence to extracellular matrix proteins. Their roles are essential for embryonic development, tumor metastasis, organ function, and proper immune cell function. Family members are heterodimers that contain a larger α subunit that is unique to each individual receptor and a smaller β subunit that can be shared by several receptors. Based on β subunit content, integrins are divided into the subfamilies β1, β2, and β3. The β3 subfamily contains the vitronectin receptor (αVβ3) and the platelet protein gpIIb/IIIa. The α chain of the vitronectin receptor (VNRα, CD51, integrin αV) has been reported to consist of a disulfide-linked large extracellular (125 kDa) subunit and a smaller (25 kDa) membrane-anchored subunit. The large subunit contains multiple sequences with homology to calcium-binding sites in other proteins and an RGD-dependent ligand-binding site, while the small subunit contains a transmembrane domain and a short cytoplasmic domain. VNRα has been reported to be noncovalently associated with the VNR β3-chain (CD61, gpIIIa). The VNR mediates cell adhesion to RGD-containing ligands such as vitronectin, von Willebrand factor, fibrinogen, and thrombospondin.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611012 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611012 Rev.1
引用&参考文献
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開発者向け参考資料 (3)

  1. Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem. 1990; 265(11):5938-5941. (Biology). 参考文献を見る
  2. Suzuki S, Argraves WS, Arai H. Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs. J Biol Chem. 1987; 262(29):14080-14085. (Biology). 参考文献を見る
  3. Suzuki S, Argraves WS, Pytela R. cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors. Proc Natl Acad Sci U S A. 1986; 83(22):8614-8618. (Biology). 参考文献を見る
611012 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.