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Purified Mouse Anti-CD29
Purified Mouse Anti-CD29

Western blot analysis of CD29 (Integrin β1) on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1: 10,000 diluton of the anti-CD29 antibody.

Purified Mouse Anti-CD29

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of CD29 (Integrin β1) on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1: 10,000 diluton of the anti-CD29 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

製品詳細
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BD Transduction Laboratories™
Integrin β1; platelet GPIIa; VLA β-chain
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human CD29 aa. 76-256
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)
130 kDa
250 µg/ml
AB_397840
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610467 Rev. 1
抗体の詳細
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18/CD29

Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are transmembrane heterodimers composed of α and β subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of  12 α and 9 β subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The β1 subgroup of this receptor family comprises at least six different dimer combinations. Among these combinations, α2β1 is associated with type I collagen and laminin binding and regulation, α3β1 is a receptor for laminin and epiligrin, and α5β1 is a fibronectin receptor. β1 integrins play an important role in several aspects of epidermal differentiation and  morphogenesis. Expression of the β1 subunit is regulated by growth factors such as TGF-β1. Integrin activation, which enhances binding of T cells to endothelium, is regulated in part by phosphatidylinositol 3-kinase. Focal adhesion kinase (FAK) and paxillin have been reported to independently bind the C-terminal, cytoplasmic domain of the β1 subunit. FAK is reported to be enzymatically activated upon engagement of integrins with their ligands and paxillin is phosphorylated on tyrosine upon activation of FAK.

610467 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610467 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Cervella P, Silengo L, Pastore C, Altruda F. Human beta 1-integrin gene expression is regulated by two promoter regions. J Biol Chem. 1993; 268(7):5148-5155. (Biology). 参考文献を見る
  2. Huan Y, van Adelsberg J. Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins. J Clin Invest. 1999; 104(10):1459-1468. (Biology: Immunohistochemistry, Western blot). 参考文献を見る
  3. Ivaska J, Whelan RD, Watson R, Parker PJ. PKC epsilon controls the traffic of beta1 integrins in motile cells. EMBO J. 2002; 21(14):3608-3619. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
  4. Tang H, Hao Q, Fitzgerald T, Sasaki T, Landon EJ, Inagami T. Pyk2/CAKbeta tyrosine kinase activity-mediated angiogenesis of pulmonary vascular endothelial cells. J Biol Chem. 2002; 277(7):5441-5447. (Biology: Western blot). 参考文献を見る
  5. Yeh CH, Peng HC, Huang TF. Accutin, a new disintegrin, inhibits angiogenesis in vitro and in vivo by acting as integrin alphavbeta3 antagonist and inducing apoptosis. Blood. 1998; 92(9):3268-3276. (Biology: Flow cytometry). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610467 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.