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Western blot analysis of Casein Kinase Iε on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Casein Kinase Iε antibody.
Immunofluorescence staining of human fibroblast cells stained with the mouse anti-Casein Kinase Iε antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Casein Kinase Iε
BD Transduction Laboratories™ Purified Mouse Anti-Casein Kinase Iε
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Casein Kinase Iε (CKIε) is a product of a family of proteins involved in the regulation of various cytoplasmic and nuclear processes including DNA replication and repair. CKIε is a 47.3 kDa serine/threonine kinase that plays a role similar to previously described CKI isoforms. It phosphorylates known CKI substrates, including a CKI-specific peptide. In addition, it is inhibited by CKI-7, an inhibitor of other CKI isoforms. CKIε contains a core kinase domain of 285 amino acids which is 53-98% identical to other isoforms and is most similiar in structure to CKIδ. Since various human and yeast CKI proteins exhibit both sequence and functional similarities, CKIε may be involved in mammalian DNA metabolism.
Development References (4)
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Akashi M, Tsuchiya Y, Yoshino T, Nishida E. Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells. Mol Cell Biol. 2002; 22(6):1693-1703. (Biology: Western blot). View Reference
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Fish KJ, Cegielska A, Getman ME, Landes GM, Virshup DM. Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family. J Biol Chem. 1995; 270(25):14875-14883. (Biology). View Reference
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Kishida M, Hino Si, Michiue T, et al. Synergistic activation of the Wnt signaling pathway by Dvl and casein kinase Iepsilon. J Biol Chem. 2001; 276(35):33147-33155. (Biology: Western blot). View Reference
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Peters JM, McKay RM, McKay JP, Graff JM. Casein kinase I transduces Wnt signals. Nature. 1999; 401(6751):345-350. (Biology: Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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