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Purified Mouse Anti-Bcl-2
Purified Mouse Anti-Bcl-2

Western blot analysis of Bcl-2 (clone 7) on Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Bcl-2.

Purified Mouse Anti-Bcl-2

Immunohistochemical staining of Bcl-2 (clone 7) on Rabbit Lung

Western blot analysis of Bcl-2 (clone 7) on Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Bcl-2.

Immunohistochemical staining of Bcl-2 (clone 7) on Rabbit Lung

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Bcl-2 aa. 49-179
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
26 kDa
250 µg/ml
AB_397896
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610538 Rev. 1
抗体の詳細
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7/Bcl-2

Human hematopoietic malignancies are often characterized by gene rearrangements which deregulate gene expression. The Bcl-2 gene (B-cell leukemia/lymphoma 2) was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2α and Bcl-2β. Both are approximately 26-30kDa and differ at their C-terminal region. Bcl-2 suppresses programmed cell death (apoptosis) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610538 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610538 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Ayllon V, Cayla X, Garcia A, et al . Bcl-2 targets protein phosphatase 1 alpha to Bad. J Immunol. 2001; 166(12):7345-7352. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
  2. Decary S, Decesse JT, Ogryzko V, et al. The retinoblastoma protein binds the promoter of the survival gene bcl-2 and regulates its transcription in epithelial cells through transcription factor AP-2. Mol Cell Biol. 2002; 22(22):7877-7888. (Clone-specific: Immunofluorescence, Western blot). 参考文献を見る
  3. Kihara T, Shimohama S, Sawada H, et al. alpha 7 nicotinic receptor transduces signals to phosphatidylinositol 3-kinase to block A beta-amyloid-induced neurotoxicity. J Biol Chem. 2001; 276(17):13541-13546. (Clone-specific: Western blot). 参考文献を見る
  4. Naga Prasad SV, Laporte SA, Chamberlain D, Caron MG, Barak L, Rockman HA. Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex. J Cell Biol. 2002; 158(3):563-575. (Clone-specific: Western blot). 参考文献を見る
  5. Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-5218. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610538 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.