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Western blot analysis of Bax on a HepG2 cell lysate (Human hepatocellular carcinoma; ATCC HB-8065). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Bax antibody.
Immunofluorescence staining of BC3H1 cells (Mouse brain smooth muscle-like cells; ATCC CRL-1443).
BD Transduction Laboratories™ Purified Mouse Anti-Bax
BD Transduction Laboratories™ Purified Mouse Anti-Bax
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
関連製品
Proper development and maintenance of homeostasis within tissues is achieved through cell proliferation, differentiation, and apoptosis. Members of the Bcl-2 protein family function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Similar to other family members, Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains which allow for its homodimerization or heterodimerization with Bcl-2. Although Bax alone does not induce cell death, elevated levels of Bax accelerate apoptosis following a death signal such as cytokine deprivation or Fas/Fas ligand interaction. The ratio of Bax homodimers to Bax/Bcl-2 heterodimers is an apoptotic checkpoint. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death. Bax function is also affected by Bax Inhibitor-1 (BI-1). Although it interacts directly with Bcl-2 and not Bax, BI-1 can suppress Bax-induced apoptosis. Alternative splicing of Bax mRNA produces Bax-α, the integral membrane form, and the two cytosolic forms β and γ. These isoforms are thought to allow for differential regulation of Bax activity and localization.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Aoudjit F, Vuori K. Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cells. Oncogene. 2001; 20(36):4995-5004. (Clone-specific: Western blot). View Reference
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Kartasheva NN, Contente A, Lenz-Stoppler C, Roth J, Dobbelstein M. p53 induces the expression of its antagonist p73 Delta N, establishing an autoregulatory feedback loop. Oncogene. 2002; 21(31):4715-4727. (Clone-specific: Western blot). View Reference
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Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology). View Reference
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Rytomaa M, Lehmann K, Downward J. Matrix detachment induces caspase-dependent cytochrome c release from mitochondria: inhibition by PKB/Akt but not Raf signalling. Oncogene. 2000; 19(39):4461-4468. (Clone-specific: Western blot). View Reference
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Xu Q, Reed JC. Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast. Mol Cell. 1998; 1(3):337-346. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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