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Purified Mouse Anti- AIM-1
Purified Mouse Anti- AIM-1

Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

Purified Mouse Anti- AIM-1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

Immunofluorescence staining of human endothelial cells.

製品詳細
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BD Transduction Laboratories™
Aurora B; Aurora and Ipl1-like midbody associated protein
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Rat AIM-1 aa. 2-124
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
41 kDa
250 µg/ml
AB_398396
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611083 Rev. 2
抗体の詳細
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6/AIM-1

The mitotic phase of the cell cycle is a complex process that ensures the fidelity of chromosome segregation. During the final stage of mitosis (telophase), segregated chromosomes become less condense and nuclear membranes surround the two sets of daughter chromosomes. Simultaneously, the separation and segregation of the cytoplasm (cytokinesis) ensures complete division and formation of two identical daughter cells. Regulation of cytokinesis is poorly understood and errors in this process can lead to cell death or oncogenesis. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae Ipl1 kinase are highly homologous and are required for progression through mitosis. Their mammalian homolog AIM-1 (also known as Aurora and Ipl1-like midbody associated protein) accumulates at the G2/M interface. During late anaphase, AIM-1 is found at the equator of central spindles. However, during telophase and cytokinesis, it is found at the midbody. Although over-expression of a kinase-inactive AIM-1 mutant disrupts formation of the cleavage furrow, nuclear division is unaffected. Thus, it is thought that AIM-1 is essential for cleavage furrowing and the onset of cytokinesis.

611083 Rev. 2
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611083 Rev.2
引用&参考文献
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開発者向け参考資料 (5)

  1. Chen J, Jin S, Tahir SK. Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells. J Biol Chem. 2003; 278(7):486-490. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
  2. Lange BM, Rebollo E, Herold A, Gonzalez C. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes. EMBO J. 2002; 21(20):5364-5374. (Biology: Immunoprecipitation). 参考文献を見る
  3. Tatsuka M, Katayama H, Ota T. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res. 1998; 58(21):4811-4816. (Biology). 参考文献を見る
  4. Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M. AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 1998; 17(3):667-676. (Biology). 参考文献を見る
  5. Trinkle-Mulcahy L, Andrews PD, Wickramasinghe S, et al. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell. 2003; 14(1):107-117. (Biology: Immunofluorescence). 参考文献を見る
すべて表示する (5) 表示項目を減らす
611083 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.