Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
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The mitotic phase of the cell cycle is a complex process that ensures the fidelity of chromosome segregation. During the final stage of mitosis (telophase), segregated chromosomes become less condense and nuclear membranes surround the two sets of daughter chromosomes. Simultaneously, the separation and segregation of the cytoplasm (cytokinesis) ensures complete division and formation of two identical daughter cells. Regulation of cytokinesis is poorly understood and errors in this process can lead to cell death or oncogenesis. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae Ipl1 kinase are highly homologous and are required for progression through mitosis. Their mammalian homolog AIM-1 (also known as Aurora and Ipl1-like midbody associated protein) accumulates at the G2/M interface. During late anaphase, AIM-1 is found at the equator of central spindles. However, during telophase and cytokinesis, it is found at the midbody. Although over-expression of a kinase-inactive AIM-1 mutant disrupts formation of the cleavage furrow, nuclear division is unaffected. Thus, it is thought that AIM-1 is essential for cleavage furrowing and the onset of cytokinesis.
Development References (5)
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Chen J, Jin S, Tahir SK. Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells. J Biol Chem. 2003; 278(7):486-490. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Lange BM, Rebollo E, Herold A, Gonzalez C. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes. EMBO J. 2002; 21(20):5364-5374. (Biology: Immunoprecipitation). View Reference
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Tatsuka M, Katayama H, Ota T. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res. 1998; 58(21):4811-4816. (Biology). View Reference
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Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M. AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 1998; 17(3):667-676. (Biology). View Reference
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Trinkle-Mulcahy L, Andrews PD, Wickramasinghe S, et al. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell. 2003; 14(1):107-117. (Biology: Immunofluorescence). View Reference
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