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Purified Mouse Anti-5-Lipoxygenase
Purified Mouse Anti-5-Lipoxygenase

Western blot analysis of 5-Lipoxygenase on SL-29 lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilultion of 5-Lipoxygenase.

Purified Mouse Anti-5-Lipoxygenase

Immunofluorescence staining of A431 cells

Western blot analysis of 5-Lipoxygenase on SL-29 lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilultion of 5-Lipoxygenase.

Immunofluorescence staining of A431 cells

製品詳細
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BD Transduction Laboratories™
Chicken (QC Testing), Human, Mouse, Rat (Tested in Development)
Mouse IgG1
Human 5-Lipoxygenase aa. 442-590
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
79 kDa
250 µg/ml
AB_398018
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610694 Rev. 1
抗体の詳細
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33/5-Lipoxygenase

5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, which are important inflammatory and vasoconstrictive metabolites.  It is activated in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene, abundantly expressed in placenta, lung, and leukocytes, encodes a protein of 674 amino acids with an apparent molecular weight of 78kDa. 5-LO is a Ca2+ and ATP-dependent enzyme that translocates from the cytosol to either a nuclear or plasma membrane compartment following activation. A proline-rich domain of 5-LO (amino acids 566-577) has been identified as a binding site for the PTyr-binding protein, Grb2. This Grb2 site links tyrosine kinases with activation and redistribution of 5-LO. Furthermore, tyrosine kinase inhibitors increase the activity of 5-LO and block the enzyme's subcellular redistribution.

610694 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610694 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Hundley TR, Prasad AR, Beaven MA. Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase. J Immunol. 2001; 167(3):1629-1636. (Clone-specific: Western blot). 参考文献を見る
  2. Lepley RA, Fitzpatrick FA. 5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts with the Src homology 3 domain of Grb2 and cytoskeletal proteins. J Biol Chem. 1994; 269(39):24163-24168. (Biology). 参考文献を見る
  3. Lepley RA, Muskardin DT, Fitzpatrick FA. Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase. J Biol Chem. 1996; 271(11):6179-6184. (Biology). 参考文献を見る
  4. Matsumoto T, Funk CD, Radmark O, Hoog JO, Jornvall H, Samuelsson B. Molecular cloning and amino acid sequence of human 5-lipoxygenase. Proc Natl Acad Sci U S A. 1988; 85(1):26-30. (Biology). 参考文献を見る
  5. Zaitsu M, Hamasaki Y, Matsuo M. New induction of leukotriene A(4) hydrolase by interleukin-4 and interleukin-13 in human polymorphonuclear leukocytes. Blood. 2000 ; 96(2):601-609. (Clone-specific: Western blot). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610694 Rev. 1

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