Preparation and Storage
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ELISA Capture: The purified BVD10-40F6 antibody (Cat.No. 554493) is useful as a capture antibody for a sandwich ELISA for measuring human IL-7 protein levels. Purified BVD10-40F6 antibody can be paired with the biotinylated BVD10-11C10 antibody (Cat. No. 554494) as the detection antibody, with recombinant human IL-7 (Cat. No. 554608) as the standard. Purified BVD10-40F6 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (eg, mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.
Neutralization: The no azide/low endotoxin format (NA/LE) of BVD10-40F6 antibody, Cat. No. 554492, is recommended for neutralization of human IL-7 bioactivity. A suitable NA/LE rat IgG1 isotype control to match the BVD10-40F6 antibody is the R3-34 antibody, Cat. No, 559072.
Western Blot: The BVD10-40F6 antibody (Cat. No. 554493) has been found useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The BVD10-40F6 antibody reacts with human interleukin-7 (IL-7). The immunogen used to generate the BVD10-40F6 hybridoma was recombinant human IL-7. This is a neutralizing antibody.
Development References (2)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
