Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The TC30-28E3 antibody specifically binds to the β subunit of the mouse Interleukin-18 Receptor complex (IL-18Rβ). The mouse IL-18R complex is expressed by T cells stimulated with IL-12 alone or cultured with antigen and IL-12 to induce Th1 cells. IL-18R is constitutively expressed by NK cells. Resting mouse T cells and Th2 cells do not express detectable levels of IL-18R. The mouse IL-18R complex consists of the IL-18α chain, also known as IL-1 Receptor related protein 1 (IL-1Rrp1 or IL-R5) and the IL-18Rβ chain also known as IL-1R accessory protein-like (IL-1RAcPL or IL-R7). The cell surface IL-18Rα chain binds IL-18 with low affinity. However its interaction with the IL-18Rβ chain confers high affinity binding for the ligand. The IL-18Rβ chain is not involved in ligand binding but is responsible for the transduction of biological signals. The immunogen used to generate the TC30-28E3 hybridoma was sIL-1R7 fusion protein.
Development References (9)
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Born TL, Thomassen E, Bird TA, Sims JE. Cloning of a novel receptor subunit, AcPL, required for interleukin-18 signaling. J Biol Chem. 1998; 273(45):29445-29450. (Biology). View Reference
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Debets R, Timans JC, Churakowa T, et al. IL-18 receptors, their role in ligand binding and function: anti-IL-1RAcPL antibody, a potent antagonist of IL-18. J Immunol. 2000; 165(9):4950-4956. (Clone-specific). View Reference
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Hoshino K, Tsutsui H, Kawai T, et al. Cutting edge: generation of IL-18 receptor-deficient mice: evidence for IL-1 receptor-related protein as an essential IL-18 binding receptor. J Immunol. 1999; 162(9):5041-5044. (Biology). View Reference
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Hyodo Y, Matsui K, Hayashi N, et al. IL-18 up-regulates perforin-mediated NK activity without increasing perforin messenger RNA expression by binding to constitutively expressed IL-18 receptor. J Immunol. 1999; 162(3):1662-1668. (Biology). View Reference
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Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). View Reference
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Neumann D, Martin MU. Interleukin-12 upregulates the IL-18Rbeta chain in BALB/c thymocytes. J Interferon Cytokine Res. 2001; 21(8):635-642. (Biology). View Reference
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Tomura M, Maruo S, Mu J, et al. Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18. J Immunol. 1998; 160(8):3759-3765. (Biology). View Reference
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Xu D, Chan WL, Leung BP, et al. Selective expression and functions of interleukin 18 receptor on T helper (Th) type 1 but not Th2 cells. J Exp Med. 1998; 188(8):1485-1492. (Biology). View Reference
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Yoshimoto T, Takeda K, Tanaka T, et al. IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-gamma production. J Immunol. 1998; 161(7):3400-3407. (Biology). View Reference
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