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Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
ELISA (IL-12 p70 Detection): Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p70 antibody (Clone 9A5, Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard. For measuring mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p70 ELISA Set (Cat. No. 555256).
ELISA (IL-12 p40 Detection): Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p40/p70 antibody (Clone C15.6, Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard. For measuring mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p40 ELISA Set (Cat. No. 555165).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
Development References (4)
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D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Biology). View Reference
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Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). View Reference
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Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med. 1995; 182(5):1281-1290. (Biology). View Reference
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Wysocka M, Kubin M, Vieira LQ, et al. Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur J Immunol. 1995; 25(3):672-676. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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