Purified NA/LE Mouse Anti-Human IFN-γ

BD Pharmingen™ Purified NA/LE Mouse Anti-Human IFN-γ

Name: Purified NA/LE Mouse Anti-Human IFN-γ
Brand: BD Pharmingen™
SKU: 554547

クローン NIB42

(RUO)

製品詳細

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Recombinant human IFN-γ

Application: ELISA (Routinely Tested), Neutralization (Tested During Development)

Concentration: 1.0 mg/ml

RRID: AB_395471

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

推奨アッセイ手順

ELISA: Purified Mouse Anti-Human IFN-γ antibody (Clone NIB42, Cat. No 551221) can be useful as a capture antibody in sandwich ELISA for measuring human IFN-γ protein levels. Purified NIB42 antibody can be paired with Biotin Mouse Anti-Human IFN-γ mAb, (Clone 4S.B3, Cat. No. 554550) as the detection antibody along with recombinant human IFN-γ (Cat. No. 554617) as the standard. For measuring human IFN-γ in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IFN-γ ELISA Set (Cat. No. 555142) or BD OptEIA™ Human IFN-γ ELISA Kit II (Cat. No. 550612).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

サイズ 0.5 mg カタログ番号 554721

Recombinant Human IFN-γ RUO

サイズ 50 µg カタログ番号 554617

Purified Mouse Anti-Human IFN-γ RUO

サイズ 1 mg カタログ番号 551221

Biotin Mouse Anti-Human IFN-γ RUO

サイズ 0.5 mg カタログ番号 554550

Human IFN-γ ELISA Set RUO

サイズ 20 Plate(s) カタログ番号 555142

Human IFN-γ ELISA Kit II RUO

サイズ 2 Plate(s) カタログ番号 550612

詳細を表示

554547 Rev. 2

抗体の詳細

NIB42

The NIB42 antibody reacts with human interferon-gamma (IFN-γ). The immunogen used to generate the NIB42 hybridoma was recombinant human IFN-γ. This is a neutralizing antibody.

554547 Rev. 2

フォーマットの詳細

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

フォーマット: NA/LE

554547 Rev.2

引用＆参考文献

Development References (2)

Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology). View Reference
Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127.

554547 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Pharmingen™ Purified NA/LE Mouse Anti-Human IFN-γ

Preparation and Storage

推奨アッセイ手順

Product Notices

関連製品

Development References (2)