BD™ ELISPOT HRP Streptavidin for ELISPOT

Use with BD ELISPOT pairs

1. Perform a 1:100 dilution of SAv-HRP in Dilution Buffer [10% Fetal Bovine Serum in Phosphate-Buffered Saline (PBS)]: (dissolve 8g NaCl, 0.2 g KCl, 1.44 g Na2HPO4, 0.24 KH2PO4 and bring up with distilled water to 1 liter, adjust pH to 7.2). For optimal results, prepare diluted SAv-HRP solution immediately prior to use.

2. Complete the assay, following instructions in the BD ELISPOT Pair Technical Data Sheet.

3. After use, discard any remaining diluted SAv-HRP solution.

Warning:  Streptavidin-HRP (component 51-9000209) contains 0.004% of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Avoid breathing dust/fume/gas/mist/vapours/spray.

Wear protective gloves/protective clothing/eye protection/face protection.

Contaminated work clothing should not be allowed out of the workplace.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. ProClin is a trademark of Rohm and Haas Company.
4. Hazardous Ingredient: ProClin™ 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water.
5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.