Phospho p38 (T180/Y182) Flex Set

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BD™ Cytometric Bead Array (CBA) Phospho p38 (T180/Y182) Flex Set

Name: Phospho p38 (T180/Y182) Flex Set
Brand: BD™ Cytometric Bead Array (CBA)
SKU: 560010

(RUO)

Protocol Library

Scientific Resources

Figure 1. Example BD CBA Phospho p38 (T180/Y182) Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Phospho p38 (T180/Y182) Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and two replicates of three different sample levels from four separate experiments (inter-assay).

Linearity: An activated cell lysate was serially diluted to determine the linearity of the assay.

Figure 1. Example BD CBA Phospho p38 (T180/Y182) Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Linearity: An activated cell lysate was serially diluted to determine the linearity of the assay.

Protocol Library

Scientific Resources

製品詳細

Brand: BD™ Cytometric Bead Array (CBA)

Bead Position: B6

Assay Range: 3.9-1,000 Units/mL

Reactivity: Human (QC Testing), Mouse,Rat (Tested in Development)

Regulatory Status: RUO

RRID: AB_2869273

説明

The BD™ CBA Phospho p38 (T180/Y182) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat p38, a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-180 and tyrosine-phosphorylated at Tyr-182 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho p38 (T180/Y182) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho p38 (T180/Y182) Flex Set. The biology and function of p38 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).

調製と保管

This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.

The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

推奨アッセイ手順

The BD CBA Phospho p38 (T180/Y182) Flex Set must be used in conjunction with a BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005, 100 tests, or 560006, 500 tests) and a flow cytometer. Detailed instructions on the use of this product can be found in the manual for the BD CBA Cell Signaling Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Phospho p38 (T180/Y182) Flex Set will be 1,000 Units/mL. An example standard curve is shown in Figure 1.

The BD CBA Phospho p38 (T180/Y182) Flex Set should not be used in the same assay well with any non-BD CBA Cell Signaling Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein Flex Sets) nor with any BD CBA Total Protein Cell Signaling Flex Set reagents. For an updated assay compatibility chart for the BD CBA Cell Signaling Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 0.64 Units/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 Units/mL, n=30) + 2 standard deviations.

製品通知

ProClin is a trademark of Rohm and Haas Company.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Warning: CBA lyophilized standard contains 32.07% sodium dodecyl sulfate (w/w) and 0.01% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May be harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause an allergic skin reaction.Precautionary statements: Wear protective gloves/eye protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Dispose of contents/container in accordance with local/regional/national/international regulations.

560010 Rev. 4

構成品

説明	数量/容量	Part Number	EntrezGene ID
Phospho p38 (T180/Y182) Capture Bead B6	1 Vial(s) (1 ea)	51-9005185	N/A
Phospho p38 (T180/Y182) PE* Detection Reagent	N/A	51-9003891	N/A
Phospho p38 (T180/Y182) Standard	1 Vial(s) (1 ea)	51-9004903	N/A

560010 Rev. 4

引用＆参考文献

開発者向け参考資料 (2)

Boldt S, Kolch W. Targeting MAPK signalling: Prometheus' fire or Pandora's box. Curr Pharm Des. 2004; 10(16):1885-1905. (Biology). 参考文献を見る
Yang SH, Sharrocks AD, Whitmarsh AJ. Transcriptional regulation by the MAP kinase signaling cascades. Gene. 2003; 320:3-21. (Biology). 参考文献を見る

560010 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.