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Mouse Soluble CD62L (L-Selectin) Flex Set

Mouse Soluble CD62L (L-Selectin) Flex Set

(RUO)
Mouse Soluble CD62L (L-Selectin) Flex Set

Figure 1. Example BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Mouse Soluble CD62L (L-Selectin) Flex Set

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.  

*ND = Not Determined. Due to high levels of CD62L (L-Selectin) in serum and EDTA-plasma samples, linearity information could not be generated.

Mouse Soluble CD62L (L-Selectin) Flex Set

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

*ND = Not Determined. Due to high levels of CD62L (L-Selectin) in serum and EDTA-plasma samples, recovery information could not be generated.

Mouse Soluble CD62L (L-Selectin) Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Figure 1. Example BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.  

*ND = Not Determined. Due to high levels of CD62L (L-Selectin) in serum and EDTA-plasma samples, linearity information could not be generated.

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

*ND = Not Determined. Due to high levels of CD62L (L-Selectin) in serum and EDTA-plasma samples, recovery information could not be generated.

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

製品詳細
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BD™ Cytometric Bead Array (CBA)
E7
40-10,000 pg/mL
Mouse (QC Testing)
RUO
AB_2869339


説明

The BD™ CBA Mouse Soluble CD62L (L-Selectin) Flex Set is a bead-based immunoassay capable of measuring mouse soluble CD62L (L-Selectin) in cell culture supernatant samples. Mouse reactivity was determined by testing samples with the BD CBA Mouse Soluble CD62P (L-Selectin) Flex Set. The biology and function of CD62L (L-Selectin) has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266/558267).

調製と保管

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 10,000 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.

推奨アッセイ手順

The BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set will be 10,000 pg/mL. An example standard curve is shown in figure 1.

The BD CBA Mouse Soluble CD62L (L-Selectin) Flex Set should not be used in the same assay well with any non-BD CBA Mouse Soluble Protein Flex Set reagents (such as BD CBA Human or Rat Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Mouse Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 8.3 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.

製品通知

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
560385 Rev. 3
構成品
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説明 数量/容量 Part Number EntrezGene ID
Mouse Soluble CD62L (L-Selectin) Standard N/A 51-9006074 N/A
Mouse Soluble CD62L (L-Selectin) PE Detection Reagent 100 Tests (1 ea) 51-9006089 N/A
Mouse Soluble CD62L (L-Selectin) Capture Bead E7 100 Tests (1 ea) 51-9006091 N/A
560385 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.