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Purified Mouse IgG2a κ Isotype Control
製品詳細
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BD Pharmingen™
Mouse BALB/c IgG2a, κ
TNP-keyhole limpet hemocyanin
ELISA, Flow cytometry (Routinely Tested), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
0.5 mg/ml
AB_479656
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推奨アッセイ手順

An isotype control should be used at the same concentration as the antibody of interest.

We suggest the purified mouse IgG2a, κ myeloma protein C1.18.4 (Cat. No. 550339) or MOPC-173 (Cat. No. 554126) for use with intracellular or immunohistochemical staining applications. We also recommend Cat. No. 555571 for immunofluorescent staining of human whole blood.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553454 Rev. 14
抗体の詳細
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G155-178

The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553454 Rev. 14
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553454 Rev.14

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

23-22944-00