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Biotin Mouse Anti-Mouse H-2Kb
製品詳細
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BD Pharmingen™
Mouse (QC Testing)
Mouse BALB/c IgG2a, κ
Mouse C57BL/6 Splenocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
14972
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


調製と保管

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

A different format of the AF6-88.5 clone reactive against mouse H-2Kb is available for immunohistorical staining of acetone-fixed frozen sections, Cat. No. 550550.  Please refer to the TDS Cat. No. 550550 for more detail information. The clone AF6-88.5 is not recommended for formalin-fixed paraffin embedded sections.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553568 Rev. 12
抗体の詳細
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AF6-88.5

The AF6-88.5 antibody recognizes the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed.

553568 Rev. 12
フォーマットの詳細
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553568 Rev.12
引用&参考文献
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開発者向け参考資料 (2)

  1. Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen: Flow cytometry). 参考文献を見る
  2. Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific). 参考文献を見る
553568 Rev. 12

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.