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      FITC Mouse IgG3, κ Isotype Control
      製品詳細
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      BD Pharmingen™
      anti-KLH
      Mouse BALB/c IgG3, κ
      TNP-KLH
      Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
      0.5 mg/ml
      AB_479697
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      推奨アッセイ手順

      Note: We recommend FITC mouse IgG3 ĸ isotype control (Cat. No 555578) for immunofluorescent staining of human whole blood and Cat. No. 556658 for non-human primate blood.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      559806 Rev. 9
      抗体の詳細
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      A112-3

      The A112-3 clone is specific for keyhole limpet hemocyanin, (KLH).  TNP-KLH, the immunogen, is not expressed on human, mouse, or rat cells.  In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone A112-3 was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

      559806 Rev. 9
      フォーマットの詳細
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      559806 Rev.9

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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