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Control γ1 FITC/γ1 PE/CD45 PerCP
製品詳細
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BD Tritest™
Human
Controls
RUO (GMP)
Buffered saline with BSA and 0.1% sodium azide.


説明

Becton Dickinson Immunocytometry Systems (BDIS) Tritest ™ Control, γ1 fluorescein isothiocyanate (FITC)/γ1 phycoerythrin (PE)/CD45 peridinin chlorophyll (PerCP), is a three-color direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with BD Tritest reagents on the Becton Dickinson family of flow cytometers. The Tritest Control is used to determine the unstained lymphocyte population on a fluorescence 1 (FL1) vs fluorescence 2 (FL2) display and to determine any nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors.

BD Tritest Control is for in vitro diagnostic use with BD in vitro diagnostic Tritest reagents.

調製と保管

1. For in vitro diagnostic use.

2. Store the reagent at 2° to 8°C to maintain stability. Do not use after expiration date shown on the label.

3. Do not freeze the reagent or expose it to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Do not use the reagent if you observe any changes in appearance. Precipitation or discoloration indicates instability or deterioration.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

340385 Rev. 1
構成品
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説明 クローン アイソタイプ EntrezGene ID
CD45 PerCP 2D1 IgG1, κ N/A
Mouse IgG1 Isotype Control PE L293 IgG1, κ N/A
Mouse IgG1 Isotype Control FITC X40 IgG1, κ N/A
340385 Rev. 1
引用&参考文献
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開発者向け参考資料 (11)

  1. Centers for Disease Control. Revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997; 46:1-29. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Clinical and Laboratory Standards Institute. 2007. (Biology).
  5. Clinical and Laboratory Standards Institute. 2007. (Biology).
  6. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  7. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
  8. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  9. Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993; 160:215-218. (Biology).
  10. Nicholson J, Jones B, Hubbard M. CD4 Tlymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:598-605. (Biology).
  11. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
すべて表示する (11) 表示項目を減らす
340385 Rev. 1

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