PE Mouse Anti-Human CD200
Clone MRC OX-104 (RUO)
- Brand BD Pharmingen™
- Alternative Name OX-2; MOX1; MOX2 ; My033
- Vol. Per Test 20 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human CD200 Recombinant Protein
- Workshop No. VII 70655; IX 40
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The MRC OX-104 monoclonal antibody specifically binds to CD200. CD200 is a 40-45 kDa type 1 transmembrane glycoprotein and is also referred to as OX2. CD200 is a member of the immunoglobulin superfamily of proteins. It contains two Ig domains, a single transmembrane region and a short cytoplasmic tail. CD200 is expressed on some dendritic cell subsets, and resting and activated T- and B-cells, but not on NK cells, monocytes, granulocytes, or platelets. It has been found on a subset of CD34+ progenitor cells. Interaction of CD200 with its receptor on macrophages induces a downregulation of macrophage activity in a variety of tissues, suggesting a regulatory function.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.