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This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
In this technical bulletin, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD® AbSeq Ab-Oligos).
The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation.
BD® Flex Single-Cell Multiplexing Kits utilize an innovative antibody-oligo technology to provide higher sample throughput for single-cell library preparation, as well as improve detection and elimination of multiplet data from single-cell experiments.
This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen-expression profiling with BD Rhapsody™ single cell capture and downstream library preparation.
This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen expression profiling with BD Rhapsody™ single-cell capture and downstream library preparation.
This protocol provides instructions on cell labeling with dCODE Dextramer® and optional BD AbSeq Ab-Oligos.
This protocol describes use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) or BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793).
This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781).
This protocol details how to stain for intracellular antigens using intracellular BD® AbSeq (IC-AbSeq) Antibodies for profiling with the BD Rhapsody™ System. Each BD® IC-AbSeq Antibody is conjugated to an antibody-specific oligonucleotide barcode for profiling alongside surface protein and mRNA expression.
This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis System.
This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System
This protocol enables users to use cell surface proteins to profile cells independently of mRNA. Included here are instructions for how to generate BD® AbSeq single-cell libraries using the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System.
This protocol enables users to use cell surface proteins to profile cells independently of mRNA. Included here are instructions for how to generate BD® AbSeq single-cell libraries and Sample Tag libraries using the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System.
This protocol provides instructions on creating single-cell whole transcriptome mRNA libraries after cell capture on the BD Rhapsody™ HT Single-Cell Analysis System or the BD Rhapsody™ HT Xpress System for sequencing on various sequencers.
This protocol provides instructions on creating single-cell whole transcriptome mRNA and BD® AbSeq libraries after cell capture on the BD Rhapsody™ HT Single-Cell Analysis System or the BD Rhapsody™ HT Xpress System for sequencing on various sequencers.
This protocol provides instructions on creating single-cell whole transcriptome mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ HT Single-Cell Analysis System or the BD Rhapsody™ HT Xpress System for sequencing on various sequencers.
This protocol provides instructions on creating single-cell whole transcriptome mRNA, BD® AbSeq and Sample Tag libraries after cell capture on the BD Rhapsody™ HT Single-Cell Analysis System or the BD Rhapsody™ HT Xpress System for sequencing on various sequencers.
This protocol provides instructions on creating a single cell mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating mRNA and BD® AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD® AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on generating a single-cell ATAC library using the BD Rhapsody™ Single-Cell Analysis System.
This protocol provides instructions on sample multiplexing and generating a single-cell ATAC library using the BD Rhapsody™ Single-Cell Analysis System.
This protocol provides instructions on generating a single-cell ATAC library and whole transcriptome analysis (WTA) mRNA library with cell-capture beads using the BD Rhapsody™ Single-Cell Analysis System.
This protocol provides instructions on sample multiplexing and generating a single-cell ATAC library and whole transcriptome analysis (WTA) mRNA library with cell-capture beads using the BD Rhapsody™ Single-Cell Analysis System.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ System.
This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and BD® AbSeq libraries.
This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ System.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR and BCR libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and Sample Tag libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and BD® AbSeq libraries.
This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq and Sample Tag libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and BD® AbSeq libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR BD® AbSeq, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR and BCR libraries
TThis protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and Sample Tag libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR and BD® AbSeq libraries.
This protocol enables high-throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ System, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq and Sample Tag libraries
This guide provides detailed instructions on how to setup and run the BD Rhapsody™ Sequence Analysis Pipeline on the Seven Bridges Genomics platform or on a local server installation.
In this technical bulletin, we show how isolated nuclei can be captured and amplified on the BD Rhapsody™ Single-Cell Analysis System.
The purpose of this protocol is to provide detailed instructions on how to use single-color flow cytometry to analyze expression of oligonucleotide-conjugated antibodies with a poly(A) tail. This protocol allows rapid analysis of the expression of protein targets in the sample of interest when labelling cells with Sample Tags from the BD® Single-Cell Multiplexing Kit or with BD® AbSeq Ab-Oligos. This protocol is used prior to cDNA Synthesis
