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BD Pharmingen™ Purified Hamster Anti-Human Bcl-2
(RUO)Western blot analysis of Bcl-2. A Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with the hamster anti-human Bcl-2 antibody at concentrations of 3.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). Bcl-2 can be identified as a band of ~ 26 kDa.
BD Pharmingen™ Purified Hamster Anti-Human Bcl-2
Purified Hamster Anti-Human Bcl-2
Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.
調製と保管
Store both the hamster anti-human Bcl-2 antibody (component 51-1513GR) and the Jurkat cell lysate (component 51-16526N) undiluted at -20°C.
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
コンパニオン製品
説明 | 数量/容量 | Part Number | EntrezGene ID |
---|---|---|---|
Purified Hamster Anti-Human Bcl-2 | 50 µg (1 ea) | 51-1513GR | N/A |
Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
開発者向け参考資料 (13)
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Batistatou A, Merry DE, Korsmeyer SJ, Greene LA. Bcl-2 affects survival but not neuronal differentiation of PC12 cells. J Neurosci. 1993; 13(10):4422-4428. (Biology: Immunofluorescence, Western blot). 参考文献を見る
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Chiou SK, Rao L, White E. Bcl-2 blocks p53-dependent apoptosis. Mol Cell Biol. 1994; 14(4):2556-2563. (Biology: Western blot). 参考文献を見る
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Hockenbery D, Nuñez G, Milliman C, Schreiber RD, Korsmeyer SJ. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature. 1990; 348(6299):334-336. (Immunogen: Immunofluorescence, Western blot). 参考文献を見る
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Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 1991; 75(2):241-251. (Biology: Immunofluorescence). 参考文献を見る
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Hockenbery DM, Zutter M, Hickey W, Nahm M, Korsmeyer SJ. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death. Proc Natl Acad Sci U S A. 1991; 88(16):6961-6965. (Biology: Immunohistochemistry, Immunoprecipitation). 参考文献を見る
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Martin JM, Veis D, Korsmeyer SJ, Sugden B. Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2. J Virol. 1993; 67(6):5269-5278. (Biology: Flow cytometry, Western blot). 参考文献を見る
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McDonnell TJ, Nunez G, Platt FM, et al. Deregulated Bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M and D-expressing B-cell population. Mol Cell Biol. 1990; 10(5):1901-1907. (Biology: Western blot). 参考文献を見る
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Nguyen M, Millar DG, Yong VW, Korsmeyer SJ, Shore GC. Targeting of Bcl-2 to the mitochondrial outer membrane by a COOH-terminal signal anchor sequence. J Biol Chem. 1993; 268(34):25265-25268. (Biology: Immunofluorescence, Western blot). 参考文献を見る
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Núñez G, Merino R, Grillot D, González-García M. Bcl-2 and Bcl-x: regulatory switches for lymphoid death and survival. Immunol Today. 1994; 15(12):582-588. (Biology). 参考文献を見る
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Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
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Veis DJ, Sentman CL, Bach EA, Korsmeyer SJ. Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes. J Immunol. 1993; 151(5):2546-2554. (Biology: Flow cytometry, Western blot). 参考文献を見る
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Yang E, Zha J, Jockel J, Boise LH, Thompson CB, Korsmeyer SJ. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell. 1995; 80(2):285-291. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
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Yin XM, Oltvai ZN, Korsmeyer SJ. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature. 1994; 369(6478):321-323. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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