FITC Hamster Anti-Human Bcl-2 Set

BD Pharmingen™ FITC Hamster Anti-Human Bcl-2 Set

Name: FITC Hamster Anti-Human Bcl-2 Set
Brand: BD Pharmingen™
SKU: 554234

(RUO)

Protocol Library

Scientific Resources

Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ flow cytometry instrument. Cells were stained with the FITC hamster anti-human Bcl-2 antibody (shaded) or with a FTIC hamster IgG2 isotype control (unshaded).

Protocol Library

Scientific Resources

製品詳細

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Regulatory Status: RUO

RRID: AB_395319

説明

Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenance of tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In the final stages, the dying cells become fragmented into "apoptotic bodies" which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the cellular response to apoptotic signals. Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types. Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.

調製と保管

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

製品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

554234 Rev. 5

構成品

説明	数量/容量	Part Number	EntrezGene ID
FITC Hamster Anti-Human Bcl-2	100 Tests (1 ea)	51-15134X	N/A
FITC Hamster Isotype Control	100 Tests (1 ea)	51-66984X	N/A

554234 Rev. 5

引用＆参考文献

開発者向け参考資料 (8)

Hockenbery D, Nuñez G, Milliman C, Schreiber RD, Korsmeyer SJ. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature. 1990; 348(6299):334-336. (Immunogen: Immunofluorescence, Western blot). 参考文献を見る
Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). 参考文献を見る
Martin JM, Veis D, Korsmeyer SJ, Sugden B. Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2. J Virol. 1993; 67(6):5269-5278. (Biology). 参考文献を見る
McDonnell TJ, Nunez G, Platt FM, et al. Deregulated Bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M and D-expressing B-cell population. Mol Cell Biol. 1990; 10(5):1901-1907. (Biology: Flow cytometry, Western blot). 参考文献を見る
Reed JC, Meister L, Tanaka S, et al. Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin. Cancer Res. 1991; 51(24):6529-6538. (Biology). 参考文献を見る
Veis DJ, Sentman CL, Bach EA, Korsmeyer SJ. Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes. J Immunol. 1993; 151(5):2546-2554. (Biology). 参考文献を見る
Williams GT. Programmed cell death: apoptosis and oncogenesis. Cell. 1991; 65(7):1097-1098. (Biology). 参考文献を見る
Yang J, Liu X, Bhalla K, et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science. 1997; 275(5303):1129-1132. (Biology). 参考文献を見る

すべて表示する (8)

554234 Rev. 5

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.