BD Pharmingen™ PE Hamster Anti-Human Bcl-2 Set
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ flow cytometry instrument. Cells were stained with the PE hamster anti-human Bcl-2 antibody (shaded) or with a PE hamster IgG2 isotype control (unshaded).
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ flow cytometry instrument. Cells were stained with the PE hamster anti-human Bcl-2 antibody (shaded) or with a PE hamster IgG2 isotype control (unshaded).
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ flow cytometry instrument. Cells were stained with the PE hamster anti-human Bcl-2 antibody (shaded) or with a PE hamster IgG2 isotype control (unshaded).
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ flow cytometry instrument. Cells were stained with the PE hamster anti-human Bcl-2 antibody (shaded) or with a PE hamster IgG2 isotype control (unshaded).
説明
Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenance of tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In the final stages, the dying cells become fragmented into "apoptotic bodies" which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the cellular response to apoptotic signals. Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types. Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.
調製と保管
製品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
| 説明 | 数量/容量 | Part Number | EntrezGene ID |
|---|---|---|---|
| N/A | 100.0 | N/A | N/A |
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.