Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b (PDGFRβ) on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line were stained with Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690) and PE Rat IgG2a, κ Isotype Control (Cat. No. 553930) [Left Plot] or with Alexa Fluor™ 647 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570044/570125) and PE Rat Anti-Mouse CD140a antibody (Cat. No. 562776) [Right Plot] at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b (PDGFRβ) versus CD140a (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD140b (PDGFRβ) expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes (Left Plot) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The splenic leukocytes and cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line (Right Plot) were then stained with either Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dashed line histograms) or Alexa Fluor™ 647 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570044/570125; solid line histograms) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD140b (PDGFRβ) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b (PDGFRβ) on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line were stained with Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690) and PE Rat IgG2a, κ Isotype Control (Cat. No. 553930) [Left Plot] or with Alexa Fluor™ 647 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570044/570125) and PE Rat Anti-Mouse CD140a antibody (Cat. No. 562776) [Right Plot] at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b (PDGFRβ) versus CD140a (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD140b (PDGFRβ) expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes (Left Plot) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The splenic leukocytes and cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line (Right Plot) were then stained with either Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dashed line histograms) or Alexa Fluor™ 647 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570044/570125; solid line histograms) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD140b (PDGFRβ) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
製品詳細
BD Pharmingen™
CD140b; PDGF-R-beta; PDGFR-beta; Pdgfrb; platelet-derived growth factor receptor beta
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Recombinant Mouse PDGFRβ Extracellular Domain Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
18596
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
サイズ
0.5 mg
カタログ番号
553142
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
557690
PE Rat IgG2a, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
553930
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PE Rat Anti-Mouse CD140A RUO
サイズ
50 µg
カタログ番号
562776
570125 Rev. 1
抗体の詳細
APB5
The APB5 monoclonal antibody specifically recognizes CD140b, the Platelet-Derived Growth Factor Receptor beta chain (PDGFRβ). CD140b is a single-pass type I transmembrane glycoprotein that contains an intracellular tyrosine kinase domain. This receptor is encoded by Pdgfrb and is widely expressed on cells within embryonic tissues and cells of mesenchymal origin in the adult mouse including fibroblasts and vascular smooth muscle cells. CD140b forms homodimers or heterodimeric receptor complexes with CD140a (PDGFRα) upon binding various dimeric Platelet-Derived Growth Factors (PDGF) isoforms that lead to receptor activation. Upon activation, CD140b is autophosphorylated at multiple tyrosine sites and, in turn, initiates several signaling cascades by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. These signaling pathways regulate cellular growth and proliferation, survival, differentiation, and migration involved in the development of embryonic tissues, blood vessel formation, wound healing, and in a range of diseases including fibrotic conditions, atherosclerosis, and malignancies.
570125 Rev. 1
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
570125 Rev.1
引用＆参考文献
Development References (7)
-
Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine.. Genes Dev. 2008; 22(10):1276-312. (Biology). View Reference
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Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).. Eur J Immunol. 2019; 49(10):1457-1973. (Clone-specific). View Reference
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Cremasco V, Woodruff MC, Onder L, et al. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.. Nat Immunol. 2014; 15(10):973-81. (Clone-specific: Flow cytometry). View Reference
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Ostman A, Heldin CH. Involvement of platelet-derived growth factor in disease: development of specific antagonists.. Adv Cancer Res. 2001; 80:1-38. (Biology). View Reference
-
Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile.. J Immunol. 2016; 196(11):4760-70. (Clone-specific: Flow cytometry). View Reference
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Sano H, Sudo T, Yokode M, et al. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice.. Circulation. 2001; 103(24):2955-60. (Immunogen: Functional assay, Immunohistochemistry, Western blot). View Reference
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Sano H, Yokode M, Takakura N, et al. Study on PDGF receptor beta pathway in glomerular formation in neonate mice.. Ann N Y Acad Sci. 2001; 947:303-5. (Clone-specific: Immunohistochemistry, Western blot). View Reference
570125 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
