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Flow cytometric analysis of γδ T cell expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE Rat Anti-Pig γδ T lymphocytes (Cat. No. 561486; solid line histogram) or with a PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.
BD Pharmingen™ PE Rat Anti-Pig γδ T Lymphocytes
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
関連製品
The MAC320 antibody monoclonal antibody specifically binds to the 270- and 280-kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes.
Development References (5)
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Binns RM, Duncan IA, Powis SJ, Hutchings A, Butcher GW. Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies. Immunology. 1992 October; 77(2):219-227. (Immunogen: Cytotoxicity, Immunoprecipitation). View Reference
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Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). View Reference
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Binns RM. The Null/gamma delta TCR+ T cell family in the pig. Vet Immunol Immunopathol. 1994 October; 43(1-3):69-77. (Biology). View Reference
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Davis WC, Zuckermann FA, Hamilton MJ, et al. Analysis of monoclonal antibodies that recognize gamma delta T/null cells. Vet Immunol Immunopathol. 1998 January; 60(3-4):305-316. (Biology). View Reference
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Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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