Expression of PE anti-mouse I-A/I-E on splenocytes from positive and negative mouse strains. Mouse spleen cells from either SJL or BALB/c mice were stained simultaneously with PE anti-I-A/I-E (clone: 2G9; Cat. No. 558593), FITC anti-mouse CD45R/B220 (Clone: RA3-6B2; Cat. No. 553088) and FITC anti-mouse CD11b (Clone: M1/70; Cat. No. 553310). The right panel dot plot represents the positive BALB/c mouse splenocytes while the left panel shows the negative SJL mouse splenocytes. Mouse I-A/I-E is expressed on both B cells and macrophages and reacts with the I-Ad and I-Ed MHC class II alloantigens. Flow cytometry was performed on a BD FACSCalibur™ flow system.
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Expression of PE anti-mouse I-A/I-E on splenocytes from positive and negative mouse strains. Mouse spleen cells from either SJL or BALB/c mice were stained simultaneously with PE anti-I-A/I-E (clone: 2G9; Cat. No. 558593), FITC anti-mouse CD45R/B220 (Clone: RA3-6B2; Cat. No. 553088) and FITC anti-mouse CD11b (Clone: M1/70; Cat. No. 553310). The right panel dot plot represents the positive BALB/c mouse splenocytes while the left panel shows the negative SJL mouse splenocytes. Mouse I-A/I-E is expressed on both B cells and macrophages and reacts with the I-Ad and I-Ed MHC class II alloantigens. Flow cytometry was performed on a BD FACSCalibur™ flow system.
製品詳細
BD Pharmingen™
H-2I; I-Ad, -Ed; I-Eb and I-Ek MHC class II alloantigens; Ia Ag; MHC II
Mouse (QC Testing)
Rat DA, also known as DA/HA IgG2a, κ
BALB/c mouse epidermal Langerhans cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
14961, 14969
AB_647221
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
関連製品
PE Rat IgG2a, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
553930
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FITC Rat Anti-CD11b RUO
サイズ
0.5 mg
カタログ番号
553310
FITC Rat Anti-Mouse CD45R/B220 RUO
サイズ
0.5 mg
カタログ番号
553088
558593 Rev. 2
抗体の詳細
2G9
The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.
558593 Rev. 2
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558593 Rev.2
引用＆参考文献
Development References (1)
-
Becker D, Mohamadzadeh M, Reske K, Knop J. Increased level of intracellular MHC class II molecules in murine Langerhans cells following in vivo and in vitro administration of contact allergens. J Invest Dermatol. 1992; 99(5):545-549. (Immunogen). View Reference
558593 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
