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Purified NA/LE Mouse Anti-Human CD25 M-A251 RUO

Purified NA/LE Mouse Anti-Human CD25 

Clone  M-A251   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
  • Concentration 1.0 mg/ml
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing)  Rhesus, Cynomolgus, Baboon (Tested in Development) 
  • Application Flow cytometry (Routinely Tested) 
    Functional assay (Tested During Development) 
  • Immunogen Phytohemagglutinin stimulated human lymphocytes
  • Workshop No. IV A053
  • Entrez Gene ID 3559 
  • Storage Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells, activated lymphocytes (T and B), and monocytes. It associates with the IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.

Format
  • Format NA/LE

Other Formats →

Suggested Companion Products

Purified NA/LE Mouse IgG1 κ Isotype Control RUO
0.5 mg Cat No: 554721

FITC Goat Anti-Mouse IgG/IgM Polyclonal RUO
0.5 mg Cat No: 555988

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

Lysing Buffer RUO
100 mL Cat No: 555899

Lysing Solution 10X Concentrate RUO (GMP)
100 Cat No: 349202

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


  1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989; :1-1182.

  2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

555429 Rev. 10
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