-
Your selected country is
Japan
- Change country/language
-
抗体試薬
- フローサイトメトリー用試薬
-
ウェスタンブロッティング抗体試薬
- イムノアッセイ試薬
-
シングルセル試薬
- BD® AbSeq Assay | シングルセル試薬
- BD Rhapsody™ Accessory Kits | シングルセル試薬
- BD® Single-Cell Multiplexing Kit | シングルセル試薬
- BD Rhapsody™ Targeted mRNA Kits | シングルセル試薬
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit | シングルセル試薬
- BD Rhapsody™ TCR/BCR Profiling Assays (VDJ Assays) | シングルセル試薬
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
-
細胞機能評価のための試薬
-
顕微鏡・イメージング用試薬
-
細胞調製・分離試薬
-
- BD® AbSeq Assay | シングルセル試薬
- BD Rhapsody™ Accessory Kits | シングルセル試薬
- BD® Single-Cell Multiplexing Kit | シングルセル試薬
- BD Rhapsody™ Targeted mRNA Kits | シングルセル試薬
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit | シングルセル試薬
- BD Rhapsody™ TCR/BCR Profiling Assays (VDJ Assays) | シングルセル試薬
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- Japan (Japanese)
- Change country/language
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Flow cytometric analysis of human CD321 staining on platelets. Peripheral blood platelets were stained with either Purified Mouse Anti-Human CD321 (JAM-1) (Cat. No. 552147; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by second step staining with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).
BD Pharmingen™ Purified Mouse Anti-Human CD321 (JAM-1)
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The M.Ab.F11 monoclonal antibody specifically binds to CD321 which is also known as JAM-1 (Junctional adhesion molecule 1), Junctional adhesion molecule A (JAM-A), and F11 Receptor (F11R). CD321 is a 32-35 kDa type I transmembrane glycoprotein that includes two extracellular immunoglobulin-like domains. CD321 is expressed on platelets, leucocytes, red blood cells, endothelial cells, epithelial cells, and various cell lines. CD321 functions as an adhesion receptor molecule on platelets. It also supports the tight junction formation between endothelial cells, where it may regulate the transendothelial migration of leucocytes, and epithelial cells. M.Ab.F11 is a stimulatory antibody that can induce morphological changes, granule secretion, and aggregation in human platelets.
Development References (8)
-
Babinska A, Kedees MH, Athar H, et al. Two regions of the human platelet F11-receptor (F11R) are critical for platelet aggregation, potentiation and adhesion. Thromb Haemost. 2002; 87(4):712-721. (Biology). View Reference
-
Florian S, Sonneck K, Czerny M, et al. Detection of novel leukocyte differentiation antigens on basophils and mast cells by HLDA8 antibodies. Allergy. 2006; 61(9):1054-1062. (Clone-specific: Flow cytometry). View Reference
-
Halasz P, Fleming FE, Coulson BS. Evaluation of specificity and effects of monoclonal antibodies submitted to the Eighth Human Leucocyte Differentiation Antigen Workshop on rotavirus-cell attachment and entry. Cell Immunol. 2005; 236(1-2):179-187. (Clone-specific: Flow cytometry). View Reference
-
Horváth O, Drbel K, Angelisová P, Hilgert I, Horejsí V. Non-lineage antigens: section report. Cell Immunol. 2005; 236(1-2):42-47. (Clone-specific). View Reference
-
Kornecki E, Walkowiak B, Naik UP, Ehrlich YH. Activation of human platelets by a stimulatory monoclonal antibody. J Biol Chem. 1990; 265(17):10042-10048. (Biology). View Reference
-
Naik UP, Ehrlich YH, Kornecki E. Mechanisms of platelet activation by a stimulatory antibody: cross-linking of a novel platelet receptor for monoclonal antibody F11 with the Fc gamma RII receptor. Biochem J. 1995; 310(1):155-162. (Biology). View Reference
-
Sobocka MB, Sobocki T, Banerjee P, et al. Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation. Blood. 2000; 95(8):2600-2609. (Biology). View Reference
-
Wang F, Naik UP, Ehrlich YH, Osada S, Ohno S, Kornecki E. Stimulatory antibody-induced activation and selective translocation of protein kinase C isoenzymes in human platelets. Biochem J. 1995; 311(2):401-406. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.