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Two-color flow cytometric analysis of CD355 (CRTAM) expression on mouse spleen cells. Splenic leucocytes from a C57BL/6 mouse were stimulated overnight with immobilized Purified NA/LE Hamster Anti-Mouse CD3e (Cat. No. 553057; 10 µg/ml for plate coating) and soluble Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294; 1 µg/ml) antibodies. The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), and then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD8a (Cat. No.551162) antibody and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD355 (CRTAM) antibody (Cat. No. 566748; Right Plot) at 0.5 µg/test. The pseudocolor density plot showing the correlated expression of CD355 (CRTAM) [or Ig Isotype control staining] versus PerCP-Cy™5.5 CD8a was derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto™ II System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Rat Anti-Mouse CD355 (CRTAM)
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The 11-5 monoclonal antibody specifically recognizes MHC class I-restricted T cell-associated molecule (CRTAM) that is also known as CD355 or Cytotoxic and regulatory T-cell molecule. CD355 (CRTAM) is encoded by Crtam which belongs to the Nectin family of cell adhesion molecules within the immunoglobulin superfamily. This type I transmembrane glycoprotein contains a V-type Ig domain, followed by a C-type Ig domain, a transmembrane region, and an intracellular region that contains a PDZ-binding motif at the C terminus. CD355 (CRTAM) is transiently expressed as a homodimer on activated NK cells, NKT cells, γδ T cells, CD8+ T cells, or CD4+ T cells. Through its V-type Ig domain, CRTAM (CD355) binds to Nectin-like molecule 2 (Necl-2) and plays important roles in adhesive interactions between different cell types and in cellular migration.
Development References (5)
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Arase N, Takeuchi A, Unno M, et al. Heterotypic interaction of CRTAM with Necl2 induces cell adhesion on activated NK cells and CD8+ T cells.. Int Immunol. 2005; 17(9):1227-37. (Immunogen: Flow cytometry). View Reference
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Fuchs A, Colonna M. The role of NK cell recognition of nectin and nectin-like proteins in tumor immunosurveillance. Semin Cancer Biol. 2006; 16(5):359-366. (Biology). View Reference
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Kennedy J, Vicari AP, Saylor V, et al. A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM).. J Leukoc Biol. 2000; 67(5):725-34. (Biology). View Reference
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Takeuchi A, Badr Mel S, Miyauchi K, et al. CRTAM determines the CD4+ cytotoxic T lymphocyte lineage. J Exp Med. 2016; 213(1):123-38. (Clone-specific: Flow cytometry). View Reference
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Takeuchi A, Itoh Y, Takumi A, et al. CRTAM confers late-stage activation of CD8+ T cells to regulate retention within lymph node. J Immunol. 2009; 183(7):4220-4228. (Biology). View Reference
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