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Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Immunofluorescent Staining and Flow Cytometric Analysis: This PE conjugated antibody is recommend for the immunofluorescent staining of γc receptor-bearing cells for flow cytometry.
Neutralization: The NA/LE™ format of the 4G3 antibody (Cat. No. 554454) strongly inhibits the IL-15-induced proliferative response of mouse CTLL cells but minimally inhibits the IL-2-induced responses of CTLL cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 4G3 monoclonal antibody specifically binds to the common γ subunit (γc) shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptors also known as CD132. This receptor is expressed constitutively at low levels on most lymphocytes, myeloid cells, embryonic thymocytes and lymphoid cell lines, but not non-lymphoid cells. The γc receptor is a 75 - 80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated.
Development References (7)
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Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Clone-specific: Neutralization). View Reference
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He YW, Adkins B, Furse RK, Malek TR. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor. J Immunol. 1995; 154(4):1596-1605. (Biology). View Reference
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He YW, Malek TR. The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor. J Immunol. 1995; 155(1):9-12. (Biology). View Reference
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Kondo M, Takeshita T, Higuchi M, et al. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science. 1994; 263(5152):1453-1454. (Biology). View Reference
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Kondo M, Takeshita T, Ishii N, et al. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science. 1993; 262(5141):1874-1877. (Biology). View Reference
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Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW. Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. J Interferon Cytokine Res. 1995; 15(5):447-454. (Biology). View Reference
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Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.