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      Purified Rat Anti-Mouse CD29
      商品详情
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      BD Pharmingen™
      Integrin β1 chain
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      (C57BL/6 x DBA/2)F1 mouse bone-marrow stromal cell clone BMS2
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation, Western blot (Reported)
      0.5 mg/ml
      AB_397099
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Please note that the epitope is lost upon sulfhydryl reduction of the antigen. For IHC, we recommend the use of purified anti-mouse CD29 mAb 9EG7 mAb in our special formulation for immunohistochemistry, Cat. no. 550531.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      558741 Rev. 12
      抗体详情
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      KMI6

      The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6  thymocytes to fibronectin and laminin.

      558741 Rev. 12
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558741 Rev.12
      报价单和参考
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      View product citations for antibody "558741" on CiteAb

      研发参考 (5)

      1. Jackson Laboratory. Mouse Genome Database. Available: http://www.informatics.jax.org/ 1998, Sept. 17.
      2. Jacobsen K, Miyake K, Kincade PW, Osmond DG.. Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo. J Exp Med. 1992; 176(4):927-935. (Immunogen: Immunoprecipitation). 查看参考
      3. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). 查看参考
      4. Wadsworth SA, Chang AC, Hong MJ, Halvorson MJ, Otto S, Coligan JE. Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation. J Immunol. 1995; 154(5):2125-2133. (Clone-specific: Immunoprecipitation). 查看参考
      5. Wu X, Miyake K, Medina KL, Kincade PW, Gimble JM. Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody. Hybridoma. 1994; 13(5):409-416. (Immunogen: Immunohistochemistry, Immunoprecipitation, Western blot). 查看参考
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      558741 Rev. 12

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.