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光谱流式细胞术
概述
多年以来,流式细胞仪在可用激光器、检测器和可测量参数数量方面的功能得到了极大的扩展。与此同时,用于流式细胞术的有效荧光染料数量呈指数级增长,扩大了新发现的可能性。传统的流式细胞术仅采集由每个荧光染料的单个滤光片定义的发射光谱的离散部分,而光谱流式细胞术能够采集全光谱。通过启用自发荧光分析和提取、检测每根激光器的更多荧光染料以及扩充了panel容量,提高了光谱流式细胞术的灵活性和功能。探索光谱流式细胞术与传统流式细胞术的不同之处,以及如何设计用于光谱流式细胞术的panel。
阅读有关光谱流式细胞术如何改变荧光染料在高维研究荧光中的开发前景


光谱流式细胞术与传统流式细胞术的比较
特性 | 光谱流式细胞术 | 传统流式细胞术 |
检测器与荧光染料比值 | 检测器多于荧光染料 | 1:1 |
单个荧光染料的效率测定 | 解混 | 补偿 |
荧光染料的选择 | 取决于激光器配置 | 取决于激光器和滤光片配置 |
自发荧光提取 | 是 | 否 |
分辨高度相似的荧光染料 | 是 | 否 |
多色panel功能 | 40多种颜色 | 约28种颜色 |
那些部分保持不变?
- 工作流(样本制备、染色、采集)
- 对于解混的准确、单染色对照的需求
- Panel设计原则
光谱流式细胞仪简介
请观看下面的光谱流式细胞术视频,探索发现各种主题,例如光谱流式细胞术与传统流式细胞术的基础知识、如何采用光谱流式细胞术在每根条激光器线中使用更多荧光染料、如何采用光谱流式细胞术增加panel尺寸等等。
光谱流式细胞仪与传统流式细胞仪的基本原理
视频中说明了以下问题:
- 什么是光谱流式细胞仪?
- 传统流式细胞术与光谱流式细胞术有何异同?
- 您如何读取光谱特征?
- 光谱流式细胞术的独特之处是什么?
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自发荧光解混
该视频说明了以下问题:
- 什么是自发荧光分离?
- 自发荧光如何影响生物分辨率?
- 什么是细胞特异性自发荧光特征?
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荧光染料选择的灵活性
该视频说明了以下问题:
- 如何在传统流式细胞术或光谱流式细胞术中选择荧光染料?
- 光谱流式细胞术如何支持每条激光线使用更多的荧光染料?
- 采用光谱流式细胞术检测荧光蛋白有哪些优势?
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扩充panel容量
该视频说明了以下问题:
- 光谱流式细胞术如何分辨高度相似的荧光染料?
- 使用高度相似的荧光染料的相似性指数和指导原则是什么?
- 40色多色光谱流式细胞仪panel的设计有哪些细节?
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多色光谱流式细胞仪panel设计的工具和注意事项
光谱流式细胞术是一项成熟的技术,提高了流式细胞术的能力、灵活性和复杂性。本次网络研讨会讨论了传统流式细胞术与光谱流式细胞术之间的基本异同,并深入探讨了关于多色光谱流式细胞术panel设计原理和工具的事实与误解。
观看网络研讨会
- 光谱流式细胞仪的定义及其独特之处
- 传统流式细胞术与光谱流式细胞术共有的panel设计基本规则和工具
- 针对光谱流式细胞术的panel设计注意事项


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BD Horizon RealYellow™ 和BD Horizon RealBlue™ 试剂
了解将何种荧光染料应用于您的panel中的每个标志物,并以一种可以导致更深入的生物学见解的方式分配该染料,这有时很难完成。碧迪医疗生物科学提供新的panel设计资源来帮助完成这些选择,并提供范围广泛的抗体特异性和荧光染料,以实现灵活和简单的panel设计。我们还在开发新试剂,例如BD Horizon RealYellow™ 和BD Horizon RealBlue™ 试剂系列,该系列具有优化的发射光谱和更少的交叉激光激发。我们的目标是帮助您建立信心,以获得可靠的数据并简化获取准确结果的流程。
碧迪医疗生物科学提供一系列支持传统流式细胞术与光谱流式细胞术的流式细胞仪和分选仪
光谱流式细胞术是传统流式细胞术的一种替代的数据采集和分析策略,其优势在于可以灵活输出荧光标记,无需更改滤光片,并且能够将更多荧光标记并入一份多色样本。


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提高您的实验能力
此份40色panel基于BD FACSymphony™ A5 SE流式细胞仪创建,使用精心挑选的荧光试剂来表征人外周血中的各种免疫细胞群。
从BD FACSymphony™ A5 SE流式细胞仪手册中获取更多信息。



BD FACSymphony™ A5 SE流式细胞仪具有五个固定对准的激光器和48个检测器,可最大程度地覆盖荧光染料发射光谱。通过启用光谱解混和基于补偿的工作流,BD FACSymphony™ A5 SE流式细胞仪增加了荧光染料选择的灵活性,并允许同时分析具有相似光谱特征的荧光染料。采用自发荧光解混,光谱解混也可用于分辨关键细胞群。
BD FACSymphony™ S6细胞分选仪基于BD FACSymphony™ A5流式细胞仪,可轻松将优化的panel从分析仪迁移到分选仪上。BD FACSymphony™ S6细胞分选仪支持传统的补偿和光谱解混方法,灵活地支持多达九个空间分离的激光器和50个参数。BD FACSymphony™ S6细胞分选仪联合生物信息学分析工具和广泛的BD高参数试剂组合,共同提供了整体的解决方案,促成了高参数实验的成功完成。
仅供研究使用,不可应用于诊断或治疗程序
Alexa Fluor(AF)是Thermo Fisher Scientific的商标,Cy是Global Life Sciences Germany
GmbH或其附属公司Cytiva的商标