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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD® OMICS-One Protein Panels
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD® OMICS-One Protein Panels
- China (Chinese)
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BD Cellismo™ Data Visualization Tool是一款用于单细胞多组学数据的二次分析及可视化的软件工具。研究人员无需编程即可实现百万级数据点的系统分析。
通过BD Cellismo™ Data Visualization Tool,您可以轻松基于多种参数对样本数据进行可视化分析;根据基因表达谱进行细胞亚群划分;生成直方图、热图、点图、火山图、小提琴图、tSNE图和UMAP图;自定义图表并导出为.PNG或.SVG文件,便于科研协作与成果发表。
观看视频,了解如何使用 BD Rhapsody™ 序列分析 Pipeline, 进行初步分析,并结合BD Cellismo™ Data Visualization Tool进行二次分析。
功能特点
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直观易用: 点击即可将数据可视化,一键生成专业图表
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用户友好: 无需编程基础,轻松上手
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多组学支持:轻松探索单细胞RNA和蛋白质数据集。
无需编写任何代码或编程,即可使用BD Cellismo™ Data Visualization Tool生成与Seurat相同的数据可视化结果
使用BD Cellismo™ Data Visualization Tool(右),您无需编写任何代码,即可生成与Seurat(左)相同的数据可视化结果。BD Cellismo™ Data Visualization Tool操作界面直观,让您通过点击操作,轻松自定义图表参数(例如,点大小、颜色、不透明度)。
输入要求:
对于WTA或AbSeq数据:
- (推荐格式)来自 BD Rhapsody™序列分析 Pipeline. 的.Cellismo输出文件。
- 其他支持的输入格式:
- Market exchange(MEX)
- H5MU/H5AD
- Seurat .rds
- Market exchange(MEX)压缩格式(.zip或tar.gz)
- H5
对于ATAC数据:
- 来自BD Rhapsody™序列分析流程的Cellismo输出文件(仅支持V3及更高版本)
发布说明
最新版本:v2.0 | 发布日期:2025年10月
新增功能:
- 支持BD Rhapsody™ ATAC-Seq试剂盒
- 新增模式:ATAC_PEAKS,ATAC_GENE_ACTIVITY,ATAC_MOTIF
- 基因组浏览器,用于在基因组位置和基因的背景下查看ATAC峰值和ATAC片段
- 基序富集分析工具——查找在给定峰集中过度富集的转录因子基序,与背景峰集进行比较
- 峰值与RNA表达相关性工具——查找与RNA表达活性相关的峰值
- RNA vs ATAC热图——查看给定细胞注释的RNA表达和ATAC基因活性相关性
- 能够组合并重新分析多个ATAC数据集
- 双重细胞检测工具
- 批量校正实用工具,提供批量校正后的计数,可用于图表页面绘制
- 集成实用工具
- 新的细胞分类工具ScType,支持自定义标记基因
- 细胞类型分类工具的置信度分数注释
- 当前项目页面的操作记录
- 数据导出选项——支持R/Seurat、Scanpy(H5AD)、Muon(H5MU)、FCS和CSV格式
- RNA数据导入选项——Seurat RDS格式
- 差异分析工具中新增DESeq2方法
- 图表页面中的“已选择的细胞操作”菜单,并新增“删除细胞”和“快速差异分析”选项
- 切换到反向颜色调色板
- 双轴图上的轮廓线覆盖功能
- 项目启动选项,包括高变基因选择的类型、高变基因数目和PCA/LSI组件数量
- 能够按模式筛选生物产品下拉菜单
- 能够导出和恢复应用程序设置
更新内容:
- 将差异表达工具(Differential Expression tool)重命名为差异分析(Differential Analysis),并新增对所有ATAC模式的支持
- 细胞注释——聚类工具支持ATAC模式
- 降维实用工具支持ATAC模式
修复内容:
- t-SNE生成错误导致的降维结果不佳
- 当未检测到线粒体基因或核糖体基因时,整体QC失败
- 仅有1个原型标记时,项目启动失败
系统需求
- Windows 10或更高版本(64位)
- MacOS 14(Sonoma)或更高版本(Intel或Apple Silicon芯片
- 最低8 GB RAM
RNA/AbSeq
- 200,000细胞时需要16 GB RAM
- 1M细胞时需要64 GB RAM
仅ATAC或ATAC多组学
- 100,000细胞时需要16 GB RAM
- 500,000细胞时需要64 GB RAM
"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."
"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."
"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."
"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."
"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."
"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."
"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."
"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."
"Absolutely love Cellismo. Combined with the Seven Bridges pipeline, it is an extremely powerful tool for quick and preliminary analyses of our single-cell datasets. As a non-bioinformatician but with extensive cytometry experience, we have used Cellismo to generate UMAPs and volcano plots for differential gene expression analyses. We liked that it was not tasking at all for a new user to learn and thus has helped in generating interest in scRNAseq analyses with our user base. Hoping for continued support and regular updates."
"Absolutely love Cellismo. Combined with the Seven Bridges pipeline, it is an extremely powerful tool for quick and preliminary analyses of our single-cell datasets. As a non-bioinformatician but with extensive cytometry experience, we have used Cellismo to generate UMAPs and volcano plots for differential gene expression analyses. We liked that it was not tasking at all for a new user to learn and thus has helped in generating interest in scRNAseq analyses with our user base. Hoping for continued support and regular updates."
"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."
"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."
"The breadth of features and stability of the platform make analyzing data headache-free. As a fledgling bioinformatician, the simplicity of Cellismo has helped me a lot on my analysis journey. In-house doublet detection and quality control help, clean up data quickly and streamline workflows. Cellismo’s support team does a wonderful job at assisting with troubleshooting questions and providing guidance in navigating the platform. Overall, Cellismo is a powerful tool in single-cell sequencing analysis, and I am looking forward to seeing where the platform goes in the future."
"The breadth of features and stability of the platform make analyzing data headache-free. As a fledgling bioinformatician, the simplicity of Cellismo has helped me a lot on my analysis journey. In-house doublet detection and quality control help, clean up data quickly and streamline workflows. Cellismo’s support team does a wonderful job at assisting with troubleshooting questions and providing guidance in navigating the platform. Overall, Cellismo is a powerful tool in single-cell sequencing analysis, and I am looking forward to seeing where the platform goes in the future."
"We are studying the diversity of cancer tissues. Among these, changes in cancer cells are closely related to chromatin structure. Cellismo is well-suited for intuitively plotting data analyzed by scATAC-seq, allowing you to visualize the state of regions of interest. It also reduces analysis time and possesses sufficient specifications for performing multi-omics analysis, including scRNA-seq. Using this program is expected to yield significant insights in research fields such as cancer."
"We are studying the diversity of cancer tissues. Among these, changes in cancer cells are closely related to chromatin structure. Cellismo is well-suited for intuitively plotting data analyzed by scATAC-seq, allowing you to visualize the state of regions of interest. It also reduces analysis time and possesses sufficient specifications for performing multi-omics analysis, including scRNA-seq. Using this program is expected to yield significant insights in research fields such as cancer."
免费,但功能强大——立即体验
立即下载免费的BD Cellismo™ Data Visualization Tool。填写信息并点击提交后,系统将自动开始下载。我们将通过您提供的信息,为您推送产品的最新进展、更新和漏洞修复内容。
Windows用户:
文件下载完成后,双击.exe文件。安装过程中将显示进度动画。安装完成后程序将自动启动,并在桌面创建快捷方式。
Mac用户:
提供两种不同的安装包,您可根据您的芯片类型进行选择-Mac(Intel)或Mac(Apple M)。您可以通过以下步骤查看您的芯片类型:点击左上角的“Apple”菜单。 选择“关于本机”,在“芯片”一栏中,将显示“Intel”或“Apple”。在下面的表格中,选择适合您的系统的选项。文件下载完成后,双击.zip文件以解压出Cellismo.app。双击Cellismo.app打开。您可以将应用程序移动到您选择的文件夹中(例如,用户的“应用程序”目录)。
获取关于BD Cellismo™ Tool新功能的逐步演示,包括支持BD Rhapsody™ ATAC-Seq试剂盒、批量校正、双重细胞检测和使用新的ScType算法进行细胞分类。
获取关于BD Cellismo™ Tool功能的逐步演示,这是一个旨在帮助您轻松可视化多组学单细胞数据的全新软件工具。
了解如何将单细胞数据导入该工具。可直接拖拽并放入.cellismo、.h5mu、.h5ad或mex格式的文件。探索来自PBMC的演示数据,包括2,500个细胞、30个蛋白质和2,500个基因。
了解如何使用BD Cellismo™ Tool的套索选择工具,根据蛋白表达选择细胞。可探索显示CD3、CD4和CD45RA表达的双轴图。选择CD4 T细胞,创建新的标注,并识别CD4初始T细胞和记忆T细胞。
了解该工具中可用于数据探索的七种图形类型。使用t-SNE和UMAP进行降维。可通过调整点大小、不透明度和调色板来自定义图形。将绘图保存为SVG或PNG格式,或添加到图库中。在分析结束时,将所有图形导出为PNG格式。
了解如何使用BD Cellismo™ Tool在单细胞分析中执行质量控制。可调整滑动条来设置元数据,如线粒体百分比、每个细胞的分子数及每个细胞的生物产物数等。设置阈值,标记不需要的细胞,并应用筛选器以优化数据集。
探索BD Cellismo™ Tool的细胞类型标注功能。使用内置的CellTypist算法及人类和小鼠参考数据集。将标注结果导出为CSV文件,用于下游分析。支持基于蛋白、RNA或组合模式创建新的聚类。
了解如何使用该工具执行差异表达分析。可分析蛋白或RNA模式,选择感兴趣的细胞,并在交互式火山图中查看结果。可调整显著性水平,突出显示基因,并在降维图上探索表达情况。
为基因或蛋白构建生物产物集,包括针对PBMC细胞类型的默认集合。在热图中可视化CD45RA、CD4和CD3等标志物的联合表达。使用实用工具计算新的降维坐标。
下载免费的BD Cellismo™工具
填写您的信息并点击“提交”按钮后,将自动开始下载。
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