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      BD Rhapsody™ Express单细胞多组学分析系统

      基于微孔的高通量单细胞捕获系统，功能强大

      概述
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      概述

      BD Rhapsody™ Express单细胞多组学分析系统采用独特的温和、稳健的微孔单细胞分离技术，能够对成百上千个单细胞进行捕获和条形码标记，用于基因组和蛋白质组信息分析。与新型 BD Rhapsody™ HT Xpress 单细胞多组学分析系统, （我们用于灵活可扩展实验设计及更高通量细胞和上样量的新平台）相比，BD Rhapsody™ Express单细胞多组学分析系统适用于较小规模的实验或通量需求。此外，BD Rhapsody™ Express单细胞多组学分析系统非常适合常规使用熟悉细胞体系且无需可视化质量控制或故障排除的场景。

       

      查看 BD Rhapsody™ Express 单细胞多组学分析系统手册.获取更多信息。


      查阅 BD Rhapsody™ Express 单细胞多组学分析系统相关耗材>

       
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      功能

      BD Rhapsody™ Express单细胞多组学分析系统提供的单细胞捕获，以微孔为基础，功能强大。
      • 桌面式设备，无液流泵装置
      • 细胞在重力作用下落入微孔，处理过程温和
      • 每个BD Rhapsody™单通道微孔板包含>220,000万个分隔的微孔阵列
      • BD Rhapsody™单通道微孔板体积约为600 µL，稀释细胞悬液可直接载入微孔板，无需浓缩至极小体积
      • 无微流通道设计，无需担心细胞堵塞通道问题
      • 微孔经特殊处理，以防止细胞黏附在表面，并经过全面的质量控制
      BD Rhapsody™单细胞分析系统使用的稳定磁性条形码标记的微球可保留用于后续实验，实现实验设计灵活性。
      • BD Rhapsody™增强型细胞捕获微球可在整个工作流程中保持完整性
      • 带有cDNA分子的微球可保存数月
      • 微球可二次取样用于创建多个测序文库
      • 存档微球可合并测序以提高操作效率
      • 可轻松将存档微球与研究合作伙伴共享
      • 存档和二次取样微球有助于性能标准化和制定标准操作规程（SOP）

       

      通过储存长达12周的微球获得的数据显示基因表达高度相关性且批次效应极小
      BD Rhapsody™ Express单细胞多组学分析系统是多中心、多队列转化研究的理想选择。

      采用无需微流体的温和重力式单细胞分离技术，可提供可靠、可再现的结果

      • 技术平行样本间批次效应极小
      • 生物平行样本间批次效应极小
      • 中心间差异性极小
      • 用户间差异性极小
      • 样本偏倚极小
      • 能够整合大型数据集且无需批次效应校正

       

      技术与生物平行样本之间、不同用户和中心之间均观察到基因表达

      Performance

      The BD Rhapsody™ Single Cell Analysis System provides reliable and reproducible results making it an ideal system for translational studies.

      performance1

      The system offers superior cell capture rates and low multiplet rates with a variety of cell types as compared to other systems

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      Strong correlation of all mRNA and protein markers identifies similar T-cell populations between cartridges

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      Equivalent data from biological replicates

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      Equivalent data from subsampled beads

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      Similar proportions of each cell type are recovered from WTA and targeted analysis on subsampled beads


       

      Applications

      The BD Rhapsody™ Single-Cell Analysis System enables simultaneous measurement of surface proteins and mRNA expression, facilitating the identification of distinct subsets of regulatory T cells

      Differential gene expression and differential protein expression analysis were performed on three main Treg subsets identified based on manual gating and bivariate analysis of CD45RA and HLA-DR sorted Treg samples. Differential gene expression analysis was performed using the BD Rhapsody™ Targeted Human Immune Response Panel (399 genes). Upregulated genes (>1.25X) are shown in the heat map above. Differential protein expression of 22 proteins was performed simultaneously using BD® AbSeq Oligo-Conjugated Antibodies.

       
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      The BD Rhapsody™ Single-Cell Analysis System can be used for deep characterization of exhausted T cells

      Expression of 12 proteins and genes that are significantly upregulated at day 14 of chronic simulation of CD8+ T cells. Two populations (red and green boxes) emerged based on the expression level of markers. Co-expression patterns were discerned for CD38, CD39 and CD94. 

       
      performance1

      The BD Rhapsody™ Single-Cell Analysis System enables resolution of innate lymphoid cell heterogeneity

      Differential protein and gene expression at the single-cell level. Heterogeneity was observed in major ILC subsets.

       
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      The BD Rhapsody™ Single-Cell Analysis System enables identification of heterogeneity within CLL samples

      Clusters are well defined using simultaneous mRNA and protein analysis. Samples from healthy donors cluster similarly. CLL samples cluster separately and uniquely. 

       
      performance1

      BD Rhapsody™ Express Single-Cell Analysis System

      A powerful, high-throughput, microwell-based single-cell partitioning system

      Request a Quote
      performance1
      Resources
      Expand All Collapse All


       

      视频
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      Counting Cells with the BD Rhapsody Single-Cell Analysis System
      Capturing Single Cells with the BD Rhapsody Express Single-Cell Analysis System
      Single-Cell Labeling with BD AbSeq and BD Single-Cell Multiplexing Kit
      One Workflow: Cell Enrichment to Single Cell Transcriptome and Proteome Analysis
      BD Rhapsody Single-Cell Analysis System Post PCR1 Purifications
      Performing Quality Control on the Final Sequencing Libraries
      BD Bioinformatics Pipelines

      Overview
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      Overview

      The BD Rhapsody™ Express System enables single-cell capture and barcoding of hundreds to thousands of single cells for analysis of genomic and proteomic information, using proprietary, gentle, robust microwell-based single-cell partitioning technology. The BD Rhapsody™ Express System can be used for lower scale experiments or throughput needs versus the NEW BD Rhapsody™ HT Xpress System, our NEW platform for flexible and scalable experimental design and higher throughput cell and sample inputs. The BD Rhapsody™ Express System is also ideal for routine use with familiar cell systems that do not require visual QC or troubleshooting.

       

      Get more information from the BD Rhapsody™ Express System brochure.


      Find BD Rhapsody™ Express System consumables >

       
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      Features

      zoom-icon

      The BD Rhapsody™ Express System provides powerful microwell-based single-cell partitioning

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      • Minimal set of benchtop equipment, no fluidic pumps
      • Allows gentle treatment of cells as they settle into microwells by gravity
      • Each BD Rhapsody™ Single-Lane Cartridge contains a microwell array with >220,000 partitions
      • The BD Rhapsody™ Single-Lane Cartridge has a volume ~600 µL; dilute cell suspensions can be loaded directly into the cartridge without needing to concentrate them into very small volumes
      • No microfluidic channels are involved so clogging of channels with cells is less of a concern
      • The microwells are specially treated to prevent cells from adhering to surfaces and extensive QC is performed
      High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.
      zoom-icon

      Stable magnetic barcoded beads used with the BD Rhapsody™ System can be retained for later experimentation allowing for flexibility with experimental design

      High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.
      zoom-icon
      • BD Rhapsody™ Enhanced Cell Capture Beads remain intact throughout the workflow
      • Beads with cDNA molecules can be stored for several monthss
      • Beads can be subsampled for creation of multiple sequencing libraries
      • Archived beads can be pooled and sequenced together for operational efficiency
      • Archived beads can be shared easily with research partners
      • Archived, subsampled beads can help standardize performance and develop SOPs

      High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.

      High correlation in gene expression seen between technical and biological replicates and between users and sites.
      zoom-icon

      The BD Rhapsody™ Express System is the ideal choice for translational, multi-center, multi-cohort studies

      High correlation in gene expression seen between technical and biological replicates and between users and sites.
      zoom-icon

      Reliable, reproducible results from gentle, gravity-based single-cell partitioning without microfluidics

      • Minimal batch effects with technical replicates
      • Minimal batch effects with biological replicates
      • Minimal site-to-site variability
      • Minimal user-to-user variability
      • Minimum sample biasinimum sample bias
      • Ability to integrate large data sets without correction for batch effects

      High correlation in gene expression seen between technical and biological replicates and between users and sites.

      Performance

      The BD Rhapsody™ Single Cell Analysis System provides reliable and reproducible results making it an ideal system for translational studies.

      performance1

      The system offers superior cell capture rates and low multiplet rates with a variety of cell types as compared to other systems

      performance1

      Strong correlation of all mRNA and protein markers identifies similar T-cell populations between cartridges

      performance1

      Equivalent data from biological replicates

      performance1

      Equivalent data from subsampled beads

      performance1

      Similar proportions of each cell type are recovered from WTA and targeted analysis on subsampled beads


       

      Applications

      The BD Rhapsody™ Single-Cell Analysis System enables simultaneous measurement of surface proteins and mRNA expression, facilitating the identification of distinct subsets of regulatory T cells

      Differential gene expression and differential protein expression analysis were performed on three main Treg subsets identified based on manual gating and bivariate analysis of CD45RA and HLA-DR sorted Treg samples. Differential gene expression analysis was performed using the BD Rhapsody™ Targeted Human Immune Response Panel (399 genes). Upregulated genes (>1.25X) are shown in the heat map above. Differential protein expression of 22 proteins was performed simultaneously using BD® AbSeq Oligo-Conjugated Antibodies.

       
      performance1

      The BD Rhapsody™ Single-Cell Analysis System can be used for deep characterization of exhausted T cells

      Expression of 12 proteins and genes that are significantly upregulated at day 14 of chronic simulation of CD8+ T cells. Two populations (red and green boxes) emerged based on the expression level of markers. Co-expression patterns were discerned for CD38, CD39 and CD94. 

       
      performance1

      The BD Rhapsody™ Single-Cell Analysis System enables resolution of innate lymphoid cell heterogeneity

      Differential protein and gene expression at the single-cell level. Heterogeneity was observed in major ILC subsets.

       
      performance1

      The BD Rhapsody™ Single-Cell Analysis System enables identification of heterogeneity within CLL samples

      Clusters are well defined using simultaneous mRNA and protein analysis. Samples from healthy donors cluster similarly. CLL samples cluster separately and uniquely. 

       
      performance1

      BD Rhapsody™ Express Single-Cell Analysis System

      A powerful, high-throughput, microwell-based single-cell partitioning system

      Request a Quote
      performance1
      Resources
      Expand All Collapse All


       

      Videos
      Down Arrow Up Arrow
      Counting Cells with the BD Rhapsody Single-Cell Analysis System
      Capturing Single Cells with the BD Rhapsody Express Single-Cell Analysis System
      Single-Cell Labeling with BD AbSeq and BD Single-Cell Multiplexing Kit
      One Workflow: Cell Enrichment to Single Cell Transcriptome and Proteome Analysis
      BD Rhapsody Single-Cell Analysis System Post PCR1 Purifications
      Performing Quality Control on the Final Sequencing Libraries
      BD Bioinformatics Pipelines

      仅供研究使用，不用于诊疗程序。

      并不确保显示结果，且结果并不具有代表性。

      Dynabeads是Thermo Fisher Scientific的商标。