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      Purified Rat Anti-Mouse PNAd Carbohydrate Epitope
      商品详情
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      BD Pharmingen™
      CD62L Ligand
      Mouse (QC Testing)
      Rat WF, also known as Wistar Furth IgM, κ
      Collagenase-dispersed BALB/c lymph node stroma
      Immunohistochemistry-paraffin (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Immunoprecipitation, Western blot (Reported)
      0.5 mg/ml
      AB_395099
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      This antibody has been tested by immunohistochemical staining (IHC) of citrate-pretreated formalin-fixed paraffin-embedded sections (5 - 20 µg/ml) to assure specificity and reactivity. Other reported applications include IHC of acetone-fixed frozen sections, immunoprecipitation, western blot analysis, and in vitro and in vivo adhesion blocking.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      553863 Rev. 7
      抗体详情
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      MECA-79

      The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of  peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin (eg, CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo, and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.

      553863 Rev. 7
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553863 Rev.7
      报价单和参考
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      View product citations for antibody "553863" on CiteAb

      研发参考 (9)

      1. Berg EL, Robinson MK, Warnock RA, Butcher EC. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J Cell Biol. 1991; 114(2):343-349. (Clone-specific: Blocking, Immunoprecipitation). 查看参考
      2. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Biology). 查看参考
      3. Diacovo TG, Puri KD, Warnock RA, Springer TA, von Andrian UH. Platelet-mediated lymphocyte delivery to high endothelial venules. Science. 1996; 273(5272):252-255. (Clone-specific: Blocking). 查看参考
      4. Faveeuw C, Gagnerault MC, Lepault F. Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice. J Immunol. 1994; 152(12):5969-5978. (Biology). 查看参考
      5. Hemmerich S, Butcher EC, Rosen SD. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med. 1994; 180(6):2219-2226. (Clone-specific: Immunoprecipitation). 查看参考
      6. Malý P, Thall A, Petryniak B, et al. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis. Cell. 1996; 86(4):643-653. (Biology). 查看参考
      7. Michie SA, Streeter PR, Bolt PA, Butcher EC, Picker LJ. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am J Pathol. 1993; 143(6):1688-1698. (Clone-specific: Blocking). 查看参考
      8. Puri KD, Finger EB, Gaudernack G, Springer TA. Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules. J Cell Biol. 1995; 131(1):261-270. (Clone-specific: Blocking, Western blot). 查看参考
      9. Streeter PR, Rouse BT, Butcher EC. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988; 107(5):1853-1862. (Immunogen: Blocking, Immunohistochemistry). 查看参考
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      553863 Rev. 7

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.