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监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Other reported applications include in vitro T-cell activation induction of apoptosis, and immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections. IHC of formalin-fixed paraffinembedded sections is not recommended. For IHC of tissue expressing the Thy-1. alloantigen, we recommend the use of anti-mouse CD90.2 mAb 53-2.1 (Cat. no. 550543) in our special formulation for immunohistochemistry.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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The G7 antibody reacts with the Thy-1.1 and Thy-1.2 alloantigens (CD90.1 and CD90.2, respectively) on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), and neurons, but not B lymphocytes, of all mouse strains. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. Soluble G7 mAb stimulates murine T-cell proliferation and IL-2 secretion, via signalling through the TCR/CD3 complex. mAb G7 has been reported to promote apoptosis of thymocytes and CTL clones, as determined by morphological and biochemical criteria. The Thy-1-triggered thymocyte death was found to be developmentally regulated and targeted mainly within the CD4+CD8+CD3- and CD4+CD8+CD3lo cultured immature thymocytes. G7 antibody does not react with Thy-1 (CD90) on rat thymocytes.
研发参考 (10)
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Gunter KC, Malek TR, Shevach EM. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med. 1984; 159(3):716-730. (Immunogen: Activation). 查看参考
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He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Biology). 查看参考
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Hueber AO, Raposo G, Pierres M, He HT. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med. 1994; 179(3):785-796. (Clone-specific: Apoptosis). 查看参考
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Kroczek RA, Gunter KC, Germain RN, Shevach EM. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986; 322(6075):181-184. (Biology). 查看参考
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Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation). 查看参考
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Nitta H, Munger W, Wilson E. Improved in situ immunodetection of leukocytes paraffin embedded mouse spleen. Cell Vis. 1997; 4:73-80. (Clone-specific: Immunohistochemistry).
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Sugiyama E, Cantagrel A, Reno T, Stafford-Brady F, Yeh ET, Bonventre JV. Selection of T cell receptor expression mutants through the functionally linked Ly-6A. Cell Immunol. 1990; 130(2):271-280. (Clone-specific: Apoptosis). 查看参考
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Tentori L, Pardoll DM, Zuniga JC . Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3. J Immunol. 1988; 140(4):1089-1094. (Clone-specific: Activation). 查看参考
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Ucker DS, Meyers J, Obermiller PS. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol. 1992; 149(5):1583-1592. (Clone-specific: Apoptosis, Induction). 查看参考
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Williams AF, Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982; 216(4547):696-703. (Biology). 查看参考
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