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新一代激光特异性流式细胞仪研究试剂
BD Horizon RealYellow™系列和RealBlue™ 系列试剂提供明亮且低背景的激光特异性的试剂选择,能帮助您简化复杂高维的方案设计。
新系列试剂由最佳光谱定位的新一代碧迪医疗染料技术和智能引导的荧光染料选择合成技术开发,可减少与其他荧光染料共用时的荧光溢出,并大幅提高荧光染料激发效率,从而优化数据分辨率,有助于在传统补偿算法流式细胞仪和光谱算法流式细胞仪上实现灵活的方案设计,并拓展高参数研究。
BD Horizon RealBlue™ 545 (RB545) Reagents
RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:
- Minimal cross-laser excitation off the 561-nm yellow-green laser
- Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers
Download the BD Horizon RealBlue™ 545 Brochure>
![RB545 vials RB545 vials](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-545/RB545-Overview-Image-1.jpg)
RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.
Performance
BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser
![RB545 Normalized Emission Filter Parameters RB545 Normalized Emission Filter Parameters](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-545/performance/Normalized-Emission-Filter-Parameters-Line-Chart-Thumbnail.jpg)
![RB545 Spread Plots RB545 Spread Plots](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-545/performance/RB545-Spread-Plots-Thumbnail.jpg)
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems
Buffers | Results |
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer | Compatible |
CellBlox™ Blocking Buffer | Compatible |
BD Cytofix™ Fixation Buffer | Stable at least 24 hours |
1% PFA | Stable at least 24 hours |
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution | Compatible with antibody staining before and after fixation |
BD FACS™ Permeabilizing Solution II | Compatible with antibody staining before and after fixation |
BD Phosflow™ Perm III Buffer | Compatible with antibody staining before and after fixation |
EDTA and Heparin | Compatible |
BD Horizon™ Brilliant Stain Buffer (BSB) | Compatible |
BD Horizon RealBlue™ 613 (RB613) 试剂
用于高参数流式细胞仪Panel和光谱流式细胞仪Panel的新型明亮荧光染料
RB613试剂利用创新型激光特异性荧光染料,主要由488 nm蓝色激光激发,实现:
- 高亮度荧光染料,以支持检测低表达的细胞表面标志物和细胞内标志物
- 最少的561 nm黄绿色激光交叉激光激发
- 与适当检测器的经典流式细胞仪和光谱流式细胞仪兼容
下载 BD Horizon RealBlue™ 613 and BD Horizon RealYellow ™ 试剂手册>
![RB613 vials RB613 vials](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/RB613-Overview-Image.jpg)
RB613试剂可用于更高参数的流式细胞仪Panel,以提高生物学分辨率
RB613试剂能提高488 nm蓝色激光条件下Panel设计的灵活性,在数百种抗体特异性中实现明显减少的交叉激光激发
性能
BD Horizon RealBlue™ 613试剂是使用488 nm蓝色激光的流式细胞仪的最佳选择
![RB613 and PE-CF594 Normalized Emission Profile RB613 and PE-CF594 Normalized Emission Profile](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/performance/image-1/RB613-and-PE-CF594-Normalized-Emission-Profile-Thumbnail.jpg)
说明]RB613和PE-CF594荧光染料的标准化发射曲线。
![RB613 Reduced Spillover Spread RB613 Reduced Spillover Spread](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/performance/image-2/RB613-Reduced-Spillover-Spread-Thumbnail.jpg)
说明]RB613在相应黄绿色激光检测器的溢漏扩散较少
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
应用
试剂可以清楚地分辨低表达的细胞表面标志物和细胞内标志物,并具有多种特异性。
![BD Horizon™ RB613试剂可以很好地分辨CD279(PD-1)](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/applications/slide-1/image-1/RB613-Resolves-CD279-Thumbnail.jpg)
![使用BD Horizon™ RB613 FoxP3试剂检测细胞内转录因子](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/applications/slide-1/image-2/RB613-FoxP3-Intracellular-Transcription-Factor-Detection-Thumbnail.jpg)
BD Horizon RealYellow™和BD Horizon RealBlue™试剂在光谱流式细胞数Panel中一起运行时可以实现良好的分辨率。
![色光谱流式Panel](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/applications/slide-2/image-1/RY-RB-Reagents-Spectral-Flow-Cytometry-Panel-Thumbnail.jpg)
![T细胞亚群检测的圈门策略](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/applications/slide-2/image-2/22-Color-Spectral-Flow-Cytometry-Panel-Fluorochrome-Marker-Assignment-Thumbnail.jpg)
BD Horizon™ RB613试剂表现出稳定的性能,在备货型生产试剂和BD OptiBuild™ 按需生产试剂之间具有批间一致性。它们在正常的实验室照明或昏暗的核心实验室照明下均具有光稳定性。
![RB613 Stable Reagent Performance](/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-613/applications/slide-3/RB613-Stable-Reagent-Performance.jpg)
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
BD Horizon RealBlue™ RB613试剂与多种固定和破膜系统兼容
Buffers | Results |
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer | Compatible |
BD Cytofix™ Fixation Buffer | Stable at least 24 hours |
1% PFA | Stable at least 24 hours |
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution | Compatible with antibody staining before and after fixation |
BD FACS™ Permeabilizing Solution II | Compatible with antibody staining before and after fixation |
BD Phosflow™ Perm Buffer III | Compatible with antibody staining before and after fixation |
EDTA and Heparin | Compatible |
BD Horizon™ Brilliant Stain Buffer (BSB) | Compatible |
BD Horizon RealBlue™ 780(RB780)试剂-PE-Cy7的更好替代品
RB780试剂是创新型激光特异性荧光染料,主要由488 nm蓝色激光激发,可以实现:
- 最小561 nm黄绿激光交叉激发
- 高亮度荧光染料,以支持检测低表达的细胞表面标记物和细胞内标记物
- 与PE-Cy7相比,降低单核细胞背景荧光
![RB-780 video RB-780 video](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/RB-780-Video-Screenshot.jpg)
RB780试剂可用于代替补偿算法流式细胞仪上的PE-Cy7或与光谱流式细胞仪上的PE-Cy7一起使用。
RB780试剂可在适宜配置且同时具有488 nm蓝色和561 nm黄绿色激光的传统补偿算法和光谱算法细胞仪上与PE-Cy7搭配使用,以增加实验参数的数量。
性能
与PE-Cy7相比,BD Horizon™ RB780试剂可实现最小的黄绿色激光的交叉激发,并降低背景荧光
![RB-780 Emission Profile RB-780 Emission Profile](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/rb-780-performance/RB780-Emission-Profile-Thumbnail.jpg)
PE-Cy7和RB780的标准化发射光谱,其中RB780可实现最小561 nm黄绿色激光的交叉激发
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/rb-780-performance/RB780-Spread-Data-Background-Thumbnail.jpg)
用BD Horizon™ RB780人CD4(SK3)试剂对人全血进行染色,在BD FACSymphony™ A5 SE流式细胞分析仪上采集数据并进行补偿。为PE-Cy7设置了双指数标度并应用于RB780,以突出显示选定通道中荧光扩散的变化。
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/rb-780-performance/RB780-Spread-Data-Thumbnail.jpg)
BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7’ to ‘与PE-Cy7相比,BD Horizon™ RB780试剂降低了背景荧光
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
应用
RB780试剂可以分辨低表达细胞表面标记物和细胞内标记物,具有广泛的特异性。RB780试剂可在传统补偿算法流式细胞仪上代替PE-Cy7,或在光谱算法流式细胞仪上与PE-Cy7搭配使用,以满足您的特定Panel设计需求。
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/applications/slide-1/Low-Expression-Marker-thumbnail.jpg)
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/applications/slide-1/Cytokine-Marker-Thumbnail.jpg)
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/applications/slide-2/RB780-Spectral-Data-Thumbnail.jpg)
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/applications/slide-2/RB780-in-Panel-Thumbnail.jpg)
BD Horizon™ RB780试剂表现出稳定的性能,在备货型生产试剂和BD OptiBuild™ 按需生产试剂之间具有批间一致性。经证实,它们在常规的实验室照明或昏暗的核心实验室照明下均具有光稳定性。
![](/content/dam/bdb/products/reagents/research-reagents/ry-586/rb-780-assets/applications/slide-3/RB780-Lot-to-Lot-Conistency.jpg)
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
BD Horizon RealBlue™ 780试剂可与多种固定和破膜系统兼容
缓冲液 | 结果 |
BD FACS™裂解液和BD Pharm Lyse™裂解缓冲液 | 兼容 |
CellBlox™封闭缓冲液 | 兼容 |
BD Cytofix™固定缓冲液 | 至少在24小时内稳定 |
1% PFA | 至少在24小时内稳定 |
BD Cytofix/Cytoperm™固定剂和破膜剂 | 与固定前后抗体染色兼容 |
BD FACS™ 破膜剂II | 与固定前后抗体染色兼容 |
BD Phosflow™ 破膜剂III | 与固定前后抗体染色兼容 |
EDTA和肝素 | 兼容 |
BD Horizon™ Brilliant染色缓冲液(BSB) | 兼容 |
BD Horizon RealYellow™ 586 (RY586) 试剂产品介绍
BD Horizon RealYellow™ 586 (RY586) 试剂是首批上市的创新型激光特异性荧光染料,主要由561-nm黄绿激光器激发,具有以下显著优势:
- 基本没有488nm蓝激光器交叉激发。
- 明亮的激光特异性荧光染料,支持对低表达的细胞表面和细胞内标记物检测
补偿算法流式细胞术:RY586试剂荧光亮度与PE相同或更高,但交叉激光激发显著减少,可在经典流式细胞仪上代替PE使用,调整实验方案设计更简单。
光谱算法流式细胞术:RY586有着与PE显著不同的光谱特征,可在光谱流式细胞仪上与PE一起使用,拓展高参数研究。
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/RY-586-Video-Thumbnail.jpg)
Performance
Click through the slides below to view performance data.
Clean & Bright
BD Horizon RealYellow™ 586 试剂因更少的交叉激光激发可提供更高的数据分辨率
BD Horizon RealYellow™ 586 试剂可实现最少的488nm蓝激光器交叉激发,对蓝色激光通道的荧光溢出较PE显著减少,并且有着极少的背景荧光和高分辨率。
![RY586 and PE mission profile](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-1/Cobalt-Performance-Slide-1-Image-1-Thumbnail.jpg)
![RY586 and PE Filter Data](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-1/Cobalt-Performance-Slide-1-Image-2-Thumbnail.jpg)
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-1/Cobalt-Performance-Slide-1-Image-3-Thumbnail.jpg)
![Background data RY586](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-1/Cobalt-Performance-Slide-1-Image-4-Thumbnail.jpg)
Versatile Applications
BD Horizon RealYellow™ 586 试剂支持多种应用
RY586试剂支持多种特异性检测,包括低表达细胞表面标记物和细胞内标记物。RY586可替代传统流式细胞仪上的PE染料以进行更灵活的方案设计,或在有561-nm激光器的光谱流式细胞仪上与PE一起使用拓展高维分析。
![Intracellular cytokine marker RY586 data](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-2/Cobalt-Performance-Slide-2-Image-1-Thumbnail.jpg)
![Low-expression marker RY586 Data](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-2/Cobalt-Performance-Slide-2-Image-2-Thumbnail.jpg)
Spectral Flow Cytometry
BD BD Horizon RealYellow™ 试剂可用于光谱流式细胞仪
RY586可与PE一起在光谱算法流式细胞仪上使用。RY586和PE亮度相当,同时具有显著地光谱特性,能在同一光谱流式多色方案中一起使用,以检测更多低表达表面标记物和细胞内标记物,赋能高维深度分析。
![Spectral](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-3/Cobalt-Performance-Slide-3-Image-1-Thumbnail.jpg)
![RY586 Spectral Panel Data](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-3/Cobalt-Performance-Slide-3-Image-2-Thumbnail.jpg)
BD Horizon RealYellow™ 试剂性能稳定
备货型生产试剂和BD OptiBuild™ 按需生产试剂均表明RY586稳定的试剂性能和良好的批间一致性,并且RY586在标准实验室照明或昏暗的核心实验室照明下均具有耐光性。
![Background data RY586](/content/dam/bdb/products/reagents/research-reagents/ry-586/performance/slide-4/Cobalt-Performance-Slide-4-Image-1-Expanded.jpg)
RY586备货型生产试剂和BD OptiBuild™ 按需生产试剂均具有稳定的试剂性能和良好的批间一致性。
使用人CD4(SK3)RY586对人全血进行染色,然后用BD FACS™红细胞裂解液进行裂解。用小鼠CD8a(53-6.7)对小鼠脾细胞进行染色。所有检测均在BD FACSymphony™A5 SE流式细胞分析仪上进行。
BD Horizon RealYellow™ 试剂可兼容多种常见的固定破膜试剂,包括:
- BD Cytofix/Cytoperm™ 固定破膜试剂盒
- BD Pharmingen™ 转录因子缓冲液套装
- BD FACS™ 红细胞裂解液
- BD Pharm Lyse™ 裂解缓冲液
- BD Cytofix™ 固定缓冲液
- BD FACS™ 破膜溶液 II
- BD Phosflow™ Perm III
- BD Horizon™ Brilliant 染色缓冲液 (BSB)
- 1% PFA
- EDTA 和肝素
- CellBlox™ 封闭缓冲液
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
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