新一代激光特异性流式细胞仪研究试剂
BD Horizon RealYellow™系列和RealBlue™ 系列试剂提供明亮且低背景的激光特异性的试剂选择，能帮助您简化复杂高维的方案设计。
新系列试剂由最佳光谱定位的新一代碧迪医疗染料技术和智能引导的荧光染料选择合成技术开发，可减少与其他荧光染料共用时的荧光溢出，并大幅提高荧光染料激发效率，从而优化数据分辨率，有助于在传统补偿算法流式细胞仪和光谱算法流式细胞仪上实现灵活的方案设计，并拓展高参数研究。
BD Horizon RealBlue™ 545 (RB545) Reagents
RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:
- Minimal cross-laser excitation off the 561-nm yellow-green laser
- Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers
Download the BD Horizon RealBlue™ 545 Brochure>
RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.
Performance
BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser
BD Horizon RealBlue™ 780（RB780）试剂-PE-Cy7的更好替代品
RB780试剂是创新型激光特异性荧光染料，主要由488 nm蓝色激光激发，可以实现：
- 最小561 nm黄绿激光交叉激发
- 高亮度荧光染料，以支持检测低表达的细胞表面标记物和细胞内标记物
- 与PE-Cy7相比，降低单核细胞背景荧光
RB780试剂可用于代替补偿算法流式细胞仪上的PE-Cy7或与光谱流式细胞仪上的PE-Cy7一起使用。
RB780试剂可在适宜配置且同时具有488 nm蓝色和561 nm黄绿色激光的传统补偿算法和光谱算法细胞仪上与PE-Cy7搭配使用，以增加实验参数的数量。
性能
与PE-Cy7相比，BD Horizon™ RB780试剂可实现最小的黄绿色激光的交叉激发，并降低背景荧光
PE-Cy7和RB780的标准化发射光谱，其中RB780可实现最小561 nm黄绿色激光的交叉激发
用BD Horizon™ RB780人CD4（SK3）试剂对人全血进行染色，在BD FACSymphony™ A5 SE流式细胞分析仪上采集数据并进行补偿。为PE-Cy7设置了双指数标度并应用于RB780，以突出显示选定通道中荧光扩散的变化。
BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7’ to ‘与PE-Cy7相比，BD Horizon™ RB780试剂降低了背景荧光
应用
RB780试剂可以分辨低表达细胞表面标记物和细胞内标记物，具有广泛的特异性。RB780试剂可在传统补偿算法流式细胞仪上代替PE-Cy7，或在光谱算法流式细胞仪上与PE-Cy7搭配使用，以满足您的特定Panel设计需求。
BD Horizon™ RB780试剂表现出稳定的性能，在备货型生产试剂和BD OptiBuild™ 按需生产试剂之间具有批间一致性。经证实，它们在常规的实验室照明或昏暗的核心实验室照明下均具有光稳定性。
BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems
| Buffers | Results |
| BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer | Compatible |
| CellBlox™ Blocking Buffer | Compatible |
| BD Cytofix™ Fixation Buffer | Stable at least 24 hours |
| 1% PFA | Stable at least 24 hours |
| BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution | Compatible with antibody staining before and after fixation |
| BD FACS™ Permeabilizing Solution II | Compatible with antibody staining before and after fixation |
| BD Phosflow™ Perm III Buffer | Compatible with antibody staining before and after fixation |
| EDTA and Heparin | Compatible |
| BD Horizon™ Brilliant Stain Buffer (BSB) | Compatible |
BD Horizon RealBlue™ 780试剂可与多种固定和破膜系统兼容
| 缓冲液 | 结果 |
BD FACS™裂解液和BD Pharm Lyse™裂解缓冲液 | 兼容 |
| CellBlox™封闭缓冲液 | 兼容 |
| BD Cytofix™固定缓冲液 | 至少在24小时内稳定 |
| 1% PFA | 至少在24小时内稳定 |
| BD Cytofix/Cytoperm™固定剂和破膜剂 | 与固定前后抗体染色兼容 |
| BD FACS™ 破膜剂II | 与固定前后抗体染色兼容 |
| BD Phosflow™ 破膜剂III | 与固定前后抗体染色兼容 |
| EDTA和肝素 | 兼容 |
| BD Horizon™ Brilliant染色缓冲液（BSB） | 兼容 |
BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7
RB780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser to offer.
- Minimal cross-laser excitation off the 561-nm yellow-green laser
- Bright fluorochrome to support the detection of low-expression surface and intracellular markers
- Reduced monocyte background compared to PE-Cy7
Download the BD Horizon RealBlue™ 780 Reagents Brochure >
RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers
RB780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.
Performance
BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7
Normalized emission profile of PE-Cy7 and RB780 with RB780 minimal excitation off the 561-nm yellow-green laser.
Human whole blood was stained with BD Horizon™ RB780 Human CD4 (SK3) Reagent, acquired and compensated on a BD FACSymphony™ A5 SE Cell Analyzer.
BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7
BD Horizon RealYellow™ 586 (RY586) 试剂产品介绍
BD Horizon RealYellow™ 586 (RY586) 试剂是首批上市的创新型激光特异性荧光染料，主要由561-nm黄绿激光器激发，具有以下显著优势：
- 基本没有488nm蓝激光器交叉激发。
- 明亮的激光特异性荧光染料，支持对低表达的细胞表面和细胞内标记物检测
补偿算法流式细胞术：RY586试剂荧光亮度与PE相同或更高，但交叉激光激发显著减少，可在经典流式细胞仪上代替PE使用，调整实验方案设计更简单。
光谱算法流式细胞术：RY586有着与PE显著不同的光谱特征，可在光谱流式细胞仪上与PE一起使用，拓展高参数研究。
Performance
Click through the slides below to view performance data.
Clean & Bright
BD Horizon RealYellow™ 586 试剂因更少的交叉激光激发可提供更高的数据分辨率
BD Horizon RealYellow™ 586 试剂可实现最少的488nm蓝激光器交叉激发，对蓝色激光通道的荧光溢出较PE显著减少，并且有着极少的背景荧光和高分辨率。
Versatile Applications
BD Horizon RealYellow™ 586 试剂支持多种应用
RY586试剂支持多种特异性检测，包括低表达细胞表面标记物和细胞内标记物。RY586可替代传统流式细胞仪上的PE染料以进行更灵活的方案设计，或在有561-nm激光器的光谱流式细胞仪上与PE一起使用拓展高维分析。
Spectral Flow Cytometry
BD BD Horizon RealYellow™ 试剂可用于光谱流式细胞仪
RY586可与PE一起在光谱算法流式细胞仪上使用。RY586和PE亮度相当，同时具有显著地光谱特性，能在同一光谱流式多色方案中一起使用，以检测更多低表达表面标记物和细胞内标记物，赋能高维深度分析。
BD Horizon RealYellow™ 试剂性能稳定
备货型生产试剂和BD OptiBuild™ 按需生产试剂均表明RY586稳定的试剂性能和良好的批间一致性，并且RY586在标准实验室照明或昏暗的核心实验室照明下均具有耐光性。
RY586备货型生产试剂和BD OptiBuild™ 按需生产试剂均具有稳定的试剂性能和良好的批间一致性。
使用人CD4（SK3）RY586对人全血进行染色，然后用BD FACS™红细胞裂解液进行裂解。用小鼠CD8a（53-6.7）对小鼠脾细胞进行染色。所有检测均在BD FACSymphony™A5 SE流式细胞分析仪上进行。
BD Horizon RealYellow™ 试剂可兼容多种常见的固定破膜试剂，包括：
- BD Cytofix/Cytoperm™ 固定破膜试剂盒
- BD Pharmingen™ 转录因子缓冲液套装
- BD FACS™ 红细胞裂解液
- BD Pharm Lyse™ 裂解缓冲液
- BD Cytofix™ 固定缓冲液
- BD FACS™ 破膜溶液 II
- BD Phosflow™ Perm III
- BD Horizon™ Brilliant 染色缓冲液 (BSB)
- 1% PFA
- EDTA 和肝素
- CellBlox™ 封闭缓冲液
BD Horizon RealBlue™ 780 Reagents are compatible with a broad range of fixation and permeabilization systems
| Buffers | Results |
| BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer | Compatible |
| CellBlox™ Blocking Buffer | Compatible |
| BD Cytofix™ Fixation Buffer | Stable at least 24 hours |
| 1% PFA | Stable at least 24 hours |
| BD Cytofix/Cytoperm™ Fixation and permeabilization Solution | Compatible with antibody staining before and after fixation |
| BD FACS™ Permeabilizing Solution II | Compatible with antibody staining before and after fixation |
| BD Phosflow™ Perm III Buffer | Compatible with antibody staining before and after fixation |
| EDTA and Heparin | Compatible |
| BD Horizon™ Brilliant Stain Buffer (BSB) | Compatible |
Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents
BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:
- Minimal cross-laser excitation off the 488-nm blue laser
- Bright fluorescence to detect low-expression surface and intracellular markers
Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.
Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.
Performance
Click through the slides below to view performance data.
Clean & Bright
BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation
BD Horizon RealYellow™ 586 Reagents exhibit minimal cross-laser excitation off the blue laser, resulting in significantly less spillover into blue detectors than PE. At the same time, they offer minimal background and high resolution comparable to PE.
Versatile Applications
BD Horizon RealYellow™ 586 Reagents have versatile applications
RY586 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RY586 reagents can be used instead of PE on conventional flow cytometers or with PE on spectral flow cytometers equipped with the 561-nm yellow-green laser to meet your specific panel design needs.
Spectral Flow Cytometry
BD Horizon RealYellow™ Reagents can be used with spectral flow cytometry
RY586 can be used with PE on spectral flow cytometers. Since RY586 provides comparable brightness to PE, it is ideal for detecting low-expression markers. Using RY586 and PE in the same spectral flow cytometry panel enables high-dimensional analysis of more low-expression surface and intracellular markers.
Stable & Consistent
Performance stability of BD Horizon RealYellow™ Reagents RY586 offers stable reagent performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents as well as proven photostability when exposed to typical lab lighting or dimmed core lab lighting.
RY586 made-to-stock reagents and BD OptiBuild™ On-Demand Reagents show consistent performance between each other and across multiple lots.
Human whole blood was stained with human CD4 (SK3) RY586, followed by lysis with BD FACS™ Lysing Solution. Mouse splenocytes were stained with mouse CD8a 53-6.7. All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.
BD Horizon RealYellow™ Reagents are compatible with a variety of common fixation and permeabilization systems, including:
- BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit
- BD Pharmingen™ Transcription Factor Buffer Set
- BD FACS™ Lysing Solution
- BD Pharm Lyse™ Lysing Buffer
- BD Cytofix™ Fixation Buffer
- BD FACS™ Permeabilizing Solution 2
- BD Phosflow™ Perm III
- BD Horizon™ Brilliant Stain Buffer (BSB)
- 1% PFA
- EDTA and heparin
- CellBlox™ Blocking Buffer
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CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. CellBlox is a trademark of Thermo Fisher Scientific.