APC-R700 Mouse Anti-Human CD3
Clone SK7 (also known as Leu-4) (RUO)
- Brand BD Horizon™
- Concentration 25 µg/mL
- Vol. Per Test 5 µL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Thymocytes
- Workshop No. II T118; III T492
- Entrez Gene ID 916
- Storage Buffer Phosphate buffered saline (PBS) containing BSA and ProClin.
- Regulatory Status RUO
Regulatory Status Legend
The CD3 antibody, clone SK7,15,16 is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human thymocytes.
The CD3 antibody reacts with the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).
APC-R700 is a tandem fluorochrome that combines APC with R700, a proprietary organic dye. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. Due to similar excitation and emission properties, APC-R700 and Alexa Fluor® 700 cannot be used simultaneously.
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.