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FITC Mouse Anti-Pig CD3ε BB23-8E6-8C8 RUO

FITC Mouse Anti-Pig CD3ε 

Clone  BB23-8E6-8C8   (RUO)

  • Brand BD Pharmingen™
  • Concentration 0.5 mg/ml
  • Isotype Mouse BALB/c IgG2a, κ
  • Reactivity Pig (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Pig peripheral blood mononuclear cells
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The BB23-8E6-8C8 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex. It recognizes all CD4+ and most CD8+ peripheral blood T lymphocytes, most thymocytes and phytohemagglutinin-stimulated blasts, and subsets of spleen and Peyer's patch lymphocytes. BB23-8E6-8C8 is a immunoglobulin isotype switch variant of the BB23-8E6 clone. This isotype-switch variant induces a proliferative response of peripheral blood mononuclear cells. The epitope recognized by BB23-8E6 mAb was designated CD3a by the Second International Swine CD Workshop.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Suggested Companion Products

PE Mouse Anti-Pig CD4a 74-12-4 RUO
0.1 mg Cat No: 559586

Lysing Buffer RUO
100 mL Cat No: 555899

Cell Viability Solution RUO
100 Tests Cat No: 555816

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


  1. Pescovitz MD, Book BK, Aasted B. Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):261-268. View reference

  2. Pescovitz MD, Book BK, Aasted B. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):251-260. View reference

559582 Rev. 5
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