PE-CF594 Rat Anti-Mouse CD314
Clone CX5 (RUO)
- Brand BD Horizon™
- Alternative Name Klrk1; NKG2D; NKG2-D; NK cell receptor D; Natural killer cell group 2D
- Concentration 0.2 mg/ml
- Isotype Rat IgG1
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Purified Mouse NKG2D protein
- Entrez Gene ID 27007
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The CX5 monoclonal antibody specifically binds to NKG2D, also known as CD314. NKG2D is a lectin-like receptor that is detected on resting and IL-2-activated NK cells, activated CD8-positive T lymphocytes, and LPS-activated macrophages, but not on resting T cells or unstimulated macrophages. NKG2D has little homology to the other members of the NKG2 family, NKG2A, C, and E, and does not form heterodimers with CD94. On NK cells, NKG2D is an activating receptor that associates with DAP10, an adapter protein that stimulates the PI3 kinase pathway. An isoform of mouse NKG2D can also associate with the signaling adapter protein DAP12, also known as KARAP (Killer cell-Associated Receptor-Associated Polypeptide), that activates the Syk and ZAP70 tyrosine kinases. On cytotoxic T cells, NKG2D is a co-stimulatory receptor that associates with DAP10. The ligands for NKG2D include the minor histocompatibility antigen H60, MULT1 (Murine UL16-binding protein-Like Transcript 1), and the five retinoic acid-inducible proteins Rae-1α, β, γ, δ and ε. Interactions of NKG2D with its ligands are involved in the regulation of innate and immune cytotoxic responses to tumor and pathogen-infected cells and in diabetes progression in the NOD mouse. The CX5 mAb blocks the binding of NKG2D to its ligands.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
- Format PE-CF594
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 612 nm
BD Horizon™ PE-CF594 is a tandem conjugate, developed exclusively by BD Biosciences, that combines PE and CF594. PE-CF594 is a brighter alternative to PE-Texas Red®, with improved spectral characteristics. PE-CF594 reagents exhibit very consistent spillover values lot to lot, making them an ideal choice for the PE-Texas Red® detector (610/20 nm).
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- CF™ is a trademark of Biotium, Inc.
- When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: email@example.com.
- Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.