Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
関連製品
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Steriod hormones, unlike polypeptide hormones, diffuse inside the cell, bind cytoplasmic or nuclear receptors, and activate specific genes. Complete identification of such receptors would greatly increase the understanding of the celluar processes activated by steriods. EFP (Estrogen receptor Finger Protein) was identified as a result of its specific interaction with the estrogen receptor. EFP contains a zinc-finger domain, indicating that it is a DNA-binding protein. EFP transcription is regulated by estrogens and, in combination with the estrogen receptor, it may modulate the expression of estrogen-responsive genes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (1)
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Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.. Proc Natl Acad Sci U S A. 1993 ; 90((23):11117-11121. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.