PE Rat Anti-β-galactosidase

BD Pharmingen™ PE Rat Anti-β-galactosidase

Name: PE Rat Anti-β-galactosidase
Brand: BD Pharmingen™
SKU: 570543

クローン 13R4.rMAb (also known as 13R4)

(RUO)

Flow cytometric analysis of β-galactosidase expression in Escherichia coli (strain K12) β-galactosidase-transfected cells. Non-transfected cells (Left Plot) or β-galactosidase-transfected cells (Right Plot) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; dashed line histogram) or PE Rat Anti-β-galactosidase antibody (Cat. No. 570542/570543; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing β-galactosidase expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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製品詳細

Brand: BD Pharmingen™

Alternative Name: beta-gal; beta-galactosidase; lacZ; lactase; β-gal; β-galactosidase

Reactivity: Bacterial (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: E. coli β-galactosidase

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 945006

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Fixation Buffer RUO

サイズ 100 mL カタログ番号 554655

Perm/Wash Buffer RUO

サイズ 100 mL カタログ番号 554723

PE Rat IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 553925

570543 Rev. 1

抗体の詳細

13R4.rMAb

13R4.rMAb is a recombinant monoclonal antibody that specifically recognizes Escherichia coli β-galactosidase, which is also known as beta-galactosidase or beta-gal. β-galactosidase is a cytoplasmatic metalloenzyme encoded by the lacZ gene in the lac operon of E. coli. β-galactosidase catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose. E. coli β-galactosidase is often used as a tag for recombinant mammalian proteins because it is not expressed by mammalian cells. This permits easy detection and purification of β-galactosidase-tagged recombinant proteins expressed by genetically engineered mammalian cells.

570543 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

570543 Rev.1

引用＆参考文献

Development References (5)

Juers DH, Matthews BW, Huber RE. LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance.. Protein Sci. 2012; 21(12):1792-807. (Biology). View Reference
Laden JC, Philibert P, Torreilles F, Pugnière M, Martineau P. Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.. Res Microbiol. 2002; 153(7):469-74. (Biology). View Reference
Martineau P, Jones P, Winter G. Expression of an antibody fragment at high levels in the bacterial cytoplasm.. J Mol Biol. 1998; 280(1):117-27. (Immunogen: ELISA, Western blot). View Reference
Philibert P, Martineau P. Directed evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation.. Microb Cell Fact. 2004; 3(1):16. (Clone-specific: Functional assay). View Reference
Sibler AP, Courtête J, Muller CD, Zeder-Lutz G, Weiss E. Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells.. FEBS J. 2005; 272(11):2878-91. (Clone-specific: Fluorescence activated cell sorting, Intracellular Staining/Flow Cytometry, Western blot). View Reference

すべて表示する (5)

570543 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.