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PE-CF594 Mouse Anti-Human PD-L2 (CD273)
PE-CF594 Mouse Anti-Human PD-L2 (CD273)
Flow cytometric analysis of PD-L2 (CD273) expression on viable Human monocyte-derived mature dendritic cells.  Human monocyte-derived mature dendritic cells were stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; dashed line histogram) or BD Horizon™ PE-CF594 Mouse Anti-Human PD-L2 (CD273) antibody (Cat. No. 569834/569909; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing PD-L2 (CD273) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of PD-L2 (CD273) expression on viable Human monocyte-derived mature dendritic cells.  Human monocyte-derived mature dendritic cells were stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; dashed line histogram) or BD Horizon™ PE-CF594 Mouse Anti-Human PD-L2 (CD273) antibody (Cat. No. 569834/569909; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing PD-L2 (CD273) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
製品詳細
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BD Horizon™
CD273; PD-L2; PDL2; PDCD1LG2; B7-DC; B7DC
Human (QC Testing)
Mouse IgG2a, κ
Human PD-L2 cDNA
Flow cytometry (Routinely Tested)
5 µl/test
80380
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  8. CF™ is a trademark of Biotium, Inc.
  9. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
  10. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  11. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
  12. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  13. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  14. For U.S. patents that may apply, see bd.com/patents.
569834 Rev. 1
抗体の詳細
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24F.10C12

The 24F.10C12 monoclonal antibody specifically recognizes Programmed Death Ligand 2 (PD-L2, PDL2) which is also known as CD273, and B7-DC (B7DC). PD-L2 (CD273) is a type 1 transmembrane glycoprotein that is encoded by PDCD1LG2 (programmed cell death 1 ligand 2) which belongs to the B7 family within the  Ig superfamily. PD-L2 (CD273) serves as a ligand for CD279, the Programmed Death 1 (PD-1) receptor. CD273 is expressed on dendritic cells, activated monocytes, medullary thymic epithelial cells, placental trophoblasts, and myocardial endothelium. PD-L2 (CD273) can function as a coinhibitor of T cell functions by binding to and signaling through CD279 (PD-1). PD-L2 (CD273) can also reportedly act through another receptor to costimulate T cell activation and cytokine production. The 24F.10C12 antibody can reportedly block the binding of PD-L2 (CD273) to CD279 (PD-1).

569834 Rev. 1
フォーマットの詳細
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PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
569834 Rev.1
引用&参考文献
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Development References (4)

  1. Brown JA, Dorfman DM, Ma FR, et al. Blockade of programmed death-1 ligand on dendritic cells enhances T cell activation and cytokine production. J Immunol. 2003; 170:1257-1266. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunohistochemistry). View Reference
  2. Messal N, Serriari NE, Pastor S, Nunès JA, Olive D. PD-L2 is expressed on activated human T cells and regulates their function.. Mol Immunol. 2011; 48(15-16):2214-9. (Clone-specific: Flow cytometry, Stimulation). View Reference
  3. Rodig N, Ryan T, Allen JA, et al. Endothelial expression of PD-L1 and PD-L2 down-regulates CD8+ T cell activation and cytolysis.. Eur J Immunol. 2003; 33(11):3117-26. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
  4. Tavukcuoglu E, Horzum U, Yilmaz KB, Esendagli G. PD-L2+ wound zone macrophage-like cells display M1/M2-mixed activation and restrain the effector Th1 responses.. Immunol Cell Biol. 2020; 98(2):152-164. (Clone-specific: Flow cytometry). View Reference
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569834 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.