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Preparation and Storage
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
- For U.S. patents that may apply, see bd.com/patents.
関連製品
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The A7R34 monoclonal antibody specifically binds to mouse CD127 which is also known as the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological functions of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. CD127 is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, the earliest thymocyte progenitors, CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, naïve and memory T lymphocytes, γδ TCR+ T cells, thymic NK cells, and bone marrow stromal cells. CD127 is downregulated upon activation of naïve T cells and CD127 re-expression identifies effector cells that subsequently differentiate into memory T cells. The A7R34 antibody blocks IL-7 binding to CD127. This antibody has been used in both in vitro and in vivo studies to define the biological roles played by CD127.
Development References (4)
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Brown VI, Hulitt J, Fish J, et al. Thymic stromal-derived lymphopoietin induces proliferation of pre-B leukemia and antagonizes mTOR inhibitors, suggesting a role for interleukin-7Ralpha signaling.. Cancer Res. 2007; 67(20):9963-70. (Clone-specific: Inhibition, Neutralization). View Reference
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Hikida M, Nakayama Y, Yamashita Y, Kumazawa Y, Nishikawa SI, Ohmori H. Expression of recombination activating genes in germinal center B cells: involvement of interleukin 7 (IL-7) and the IL-7 receptor.. J Exp Med. 1998; 188(2):365-72. (Clone-specific: Functional assay, Inhibition, In vivo exacerbation). View Reference
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Hozumi K, Kondo M, Nozaki H, et al. Implication of the common gamma chain of the IL-7 receptor in intrathymic development of pro-T cells. Int Immunol. 1994; 6(9):1451-1454. (Clone-specific: Functional assay, Inhibition). View Reference
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Sudo T, Nishikawa S, Ohno N, et al. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A. 1993; 90(19):9125-9129. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, In vivo exacerbation, Neutralization). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
