Multicolor flow cytometric analysis of TCF-7/TCF-1 expression in Human lymphocytes or Mouse splenocytes. Bivariate pseudocolor density plots showing the correlated expression of TCF-7/TCF-1 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific. Top Plots - Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with APC Mouse Anti-Human CD4 antibody (Cat. No. 555349/561840/561841) and either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 567018; Right Plot) at 0.06 μg/test. Bottom Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 561091/553051) and either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Left Plot) or Alexa Fluor™ 488 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.06 μg/test.
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Multicolor flow cytometric analysis of TCF-7/TCF-1 expression in Human lymphocytes or Mouse splenocytes. Bivariate pseudocolor density plots showing the correlated expression of TCF-7/TCF-1 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific. Top Plots - Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with APC Mouse Anti-Human CD4 antibody (Cat. No. 555349/561840/561841) and either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 567018; Right Plot) at 0.06 μg/test. Bottom Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 561091/553051) and either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Left Plot) or Alexa Fluor™ 488 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.06 μg/test.
製品詳細
BD Pharmingen™
TCF-1; TCF-7; Tcf1; Tcf7; transcription factor 7
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Mouse TCF-7 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
6932, 21414
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
Transcription Factor Buffer Set RUO
サイズ
100 Tests
カタログ番号
562574
Alexa Fluor® 488 Mouse IgG1 κ Isotype Control RUO
サイズ
50 µg
カタログ番号
565572
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567018 Rev. 2
抗体の詳細
S33-966
The S33-966 monoclonal antibody specifically binds to TCF-7 (Transcription factor 7) which is also known as TCF-1 (T-cell factor 1). TCF-7 is a member of the High Mobility Group (HMG) DNA binding protein family of transcription factors. TCF-7 is expressed in thymocytes, T lymphocytes and proliferating epithelial cells. TCF-7 is expressed in the earliest T cell precursors and serves as a critical transcriptional regulator that is involved in the differentiation of thymocytes to become mature T lymphocytes. TCF-7 is necessary for the survival of immature CD4+CD8+ thymocytes. TCF-7 can also promote the differentiation of mature peripheral T cells to become Th2-like cells. TCF-7 may also function as a transcriptional repressor of certain target genes including CTNNB1 and TCF7L2. The S33-966 antibody crossreacts with human TCF-7.
567018 Rev. 2
フォーマットの詳細
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
567018 Rev.2
引用＆参考文献
Development References (5)
-
Chen Z, Ji Z, Ngiow SF, et al. TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision.. Immunity. 2019; 51(5):840-855.e5. (Clone-specific: Flow cytometry). View Reference
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Germar K, Dose M, Konstantinou T, et al. T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling. Proc Natl Acad Sci U S A. 2011; 108(50):20060-20065. (Biology). View Reference
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Johnson JL, Georgakilas G, Petrovic J, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells.. Immunity. 2018; 48(2):243-257.e10. (Clone-specific: Flow cytometry). View Reference
-
Weber BN, Chi AW, Chavez A, et al. A critical role for TCF-1 in T-lineage specification and differentiation. Nature. 2011; 476(7358):63-68. (Biology). View Reference
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Yu Q, Sharma A, Oh SY, et al. T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-γ. Nat Immunol. 2009; 10(9):992-999. (Biology). View Reference
567018 Rev. 2
Please refer to Support Documents for Quality Certificates
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
