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      PE Mouse Anti-Human Alpha-fetoprotein
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      PE Mouse Anti-Human Alpha-fetoprotein
      Flow cytometric analysis of AFP expression in human liver hepatocellular carcinoma cells.  Human liver hepatocellular carcinoma cells Hep G2 (ATCC®, HB-8065™) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527), fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG2a, κ isotype control (dashed line, Cat. No. 558595) or PE Mouse anti-Human Alpha-fetoprotein monoclonal antibody (solid line) at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of HepG2 cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system
      PE Mouse Anti-Human Alpha-fetoprotein
      Flow cytometric analysis of AFP expression in human liver hepatocellular carcinoma cells.  Human liver hepatocellular carcinoma cells Hep G2 (ATCC®, HB-8065™) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527), fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG2a, κ isotype control (dashed line, Cat. No. 558595) or PE Mouse anti-Human Alpha-fetoprotein monoclonal antibody (solid line) at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of HepG2 cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system
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      BD Pharmingen™
      Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP
      Human (QC Testing)
      Mouse BALB/c IgG2a
      Human Alpha-fetoprotein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2737943
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
      4. All other brands are trademarks of their respective owners.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563002 Rev. 1
      抗体の詳細
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      C3/AFP

      Alpha-fetoprotein (AFP) is a member of the albuminoid gene family which includes albumin, alpha-albumin, and vitamin D protein. Alpha-fetoprotein is an abundant plasma protein synthesized from fetal yolk sac, liver, and gastrointestinal tract during development. Similar to albumin, AFP binds and transports multiple ligands such as nickel, copper, billirubin, and fatty acids. AFP levels are low to absent in healthy adult tissues whereas elevated AFP levels in the adult can be indicative of malignancies. In particular, AFP is expressed in hepatocellular carcinoma, germ cell tumors, and metatstatic cancers of the liver. Maternal serum AFP levels can be monitored to screen for fetal abnormalities. AFP is used as a hepatic progenitor marker in hepatic differentiations from pluripotent stem cells.

      563002 Rev. 1
      フォーマットの詳細
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      563002 Rev.1
      引用＆参考文献
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      Development References (5)

      1. Duan Y, Catana A, Meng Y, et al. Differentiation and enrichment of hepatocyte-like cells from human embryonic stem cells in vitro and in vivo. Stem Cells. 2007; 25(15):3058-3068. (Biology). View Reference
      2. Jozefczuk J, Prigione A, Chavez L, Adjaye J. Comparative analysis of human embryonic stem cell and induced pluripotent stem cell-derived hepatocyte-like cells reveals current drawbacks and possible strategies for improved differentiation. Stem Cells Dev. 2011; 20(7):1259-1275. (Biology). View Reference
      3. Mizejewski GJ. Alpha-fetoprotein structure and function: relevance to isoforms, epitopes, and conformational variants. Exp Biol Med (Maywood). 2001; 226(5):377-408. (Biology). View Reference
      4. Yazova AK, Goussev AI, Christiansen M, et al. Human fetal and tumor alpha-fetoproteins differ in conformationally dependent epitope variants expression. Immunol Lett. 2003; 85(3):261-270. (Clone-specific). View Reference
      5. Yazova AK, Goussev AI, Poltoranina VS, Yakimenko EF. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunol Lett. 1990; 25(4):325-330. (Clone-specific). View Reference
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      563002 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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