V450 Mouse Anti-Rat CD45RA

BD Horizon™ V450 Mouse Anti-Rat CD45RA

Name: V450 Mouse Anti-Rat CD45RA
Brand: BD Horizon™
SKU: 561626

クローン OX-33

(RUO)

Multicolor flow cytometric analysis of CD45RA expressed on rat splenocytes. Splenocytes from a Lewis rat were stained with a FITC Mouse Anti-Rat CD3 antibody (Cat. No. 559975/554832) and either a BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373, Left Panel) or a BD Horizon™ V450 Mouse Anti-Rat CD45RA antibody (Cat. No. 561626, Right Panel). Two color dot plots showing the correlated expression of CD45RA (or Ig isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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製品詳細

Brand: BD Horizon™

Alternative Name: Ptprc; Lca; Leucocyte common antigen

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Leukocyte common antigen purified from rat splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10894392

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

V450 Mouse IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 560373

FITC Mouse Anti-Rat CD3 RUO

サイズ 0.1 mg カタログ番号 559975

FITC Mouse Anti-Rat CD3 RUO

サイズ 0.5 mg カタログ番号 554832

561626 Rev. 1

抗体の詳細

OX-33

The OX-33 antibody specifically recognizes a high-molecular-weight form of CD45 found only on B lymphocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

561626 Rev. 1

フォーマットの詳細

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: V450

励起源: Violet 405 nm

最大励起波長: 405 nm

最大蛍光波長: 450 nm

561626 Rev.1

引用＆参考文献

Development References (2)

Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
Woollett GR, Barclay AN, et al. Molecular and antigenic heterogeneity of the rat leukocyte common antigen from thymocytes and T and B lymphocytes. . Eur J Immunol. 1985; 15:168-173. (Immunogen: Flow cytometry).

561626 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.