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Flow cytometric analysis of CD27 expression on Rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Rhesus whole blood was stained with either FITC Mouse Anti-Human CD27 (Cat. No. 557329/555440/560986; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555649; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.
BD Pharmingen™ FITC Mouse Anti-Human CD27
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Clone M-T271 reacts with the human form of 110 kDa disulfide-linked dimer of two polypeptide chains. CD27 molecule is a lymphocyte-specific member of the TNFR/NGFR family, and is expressed on the subset of human thymocytes and on the majority of mature T lymphocytes. This clone also cross-reacts with a major subset of peripheral blood lymphocytes and monocytes of baboon, and both rhesus and cynomolgus macaque monkeys. CD27+ cells were detected on all CD3+ lymphocytes and on a subset of CD20+ cells. The reactivity on lymphocytes and monocytes is similar to that observed with peripheral blood lymphocytes from normal human donors.
Development References (3)
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Bigler RD, Bushkin Y, Chiorazzi N. S152 (CD27). A modulating disulfide-linked T cell activation antigen. J Immunol. 1988; 141(1):21-28. (Biology). View Reference
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Bigler RD, Donat TL, Boselli CM. Definition of three epitopes of the CD27 molecule [P 120->55] present on activated normal lymphocytes. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:351-352.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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