Flow cytometric analysis of CD30 expression on YT (NK cell line) cells. YT cells were stained with either Biotin Mouse Anti-Human CD30 (Cat. No. 555828; solid line histogram) or Biotin Mouse IgG1, κ Isotype Control (Cat. No. 555747; dashed line histogram), followed by PE Streptavidin (Cat. No. 554061). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometery was performed on a BD FACScan™ system.
Flow cytometric analysis of CD30 expression on YT (NK cell line) cells. YT cells were stained with either Biotin Mouse Anti-Human CD30 (Cat. No. 555828; solid line histogram) or Biotin Mouse IgG1, κ Isotype Control (Cat. No. 555747; dashed line histogram), followed by PE Streptavidin (Cat. No. 554061). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometery was performed on a BD FACScan™ system.
製品詳細
BD Pharmingen™
TNFRSF8; TNR8; CD30L/CD153 receptor; Ki-1; D1S166E
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
III A171; IV A105; V A042
943
AB_396154
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Biotin Mouse IgG1 κ Isotype Control RUO
サイズ
100 Tests
カタログ番号
555747
PE Streptavidin RUO
サイズ
0.5 mg
カタログ番号
554061
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555828 Rev. 4
抗体の詳細
BerH8
The BerH8 monoclonal antibody specifically binds to CD30, a 120 kDa type I transmembrane glycoprotein expressed on stimulated T and B cells. CD30 is an activation marker initially identified to be expressed on Reed-Sternberg cells. It is also expressed on a few extrafollicular T and B cells located at the rim of germinal centers. CD30 serves as a cytokine receptor. It belongs to the nerve growth factor receptor/tumor necrosis factor receptor (NGFR/TNFR) superfamily and is also known as TNFRSF8. CD30 interaction with CD30 ligand (CD30L/CD153/TNFSF8) can mediate signals for proliferation, apoptosis and cytotoxicity of lymphoid cells.
555828 Rev. 4
フォーマットの詳細
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
555828 Rev.4
引用＆参考文献
Development References (7)
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Beverly PCL. Activation antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:516-524.
-
Bowen MA, Olsen KJ, Cheng L, Avila D, Podack ER. Functional effects of CD30 on a large granular lymphoma cell line, YT. Inhibition of cytotoxicity, regulation of CD28 and IL-2R, and induction of homotypic aggregation. J Immunol. 1993; 151(11):5896-5906. (Clone-specific). View Reference
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Franke AC, Jung D, Ellis TM. Characterization of the CD30L binding domain on the human CD30 molecule using anti-CD30 antibodies. Hybridoma. 2000; 19(1):43-48. (Biology). View Reference
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Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
-
Schwarting R, Stein H. Cluster report CD30. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:419-422.
555828 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
